CAJ | 학술논문

目的：观察染料木素对大鼠离体胃肠平滑肌收缩活动的影响，并探讨其作用机制。方法取大鼠胃肠平滑肌条安置在恒温灌流肌槽中，记录其收缩活动，同时做CaCl2的量效曲线。结果染料木素能降低胃肠平滑肌条的张力，减小其收缩波平均振幅。酚妥拉明、左旋-NG-硝基精氨酸、格列本脲、SQ22536可部分阻断染料木素对大鼠离体胃肠收缩活动的抑制作用。1．00 mol/L的染料木素对CaCl2的量效曲线无影响，50．0 mol/L染料木素使 CaCl2的量效曲线右移。结论染料木素对大鼠胃肠平滑肌条收缩活动具有抑制作用。
목적：관찰염료목소대대서리체위장평활기수축활동적영향，병탐토기작용궤제。방법취대서위장평활기조안치재항온관류기조중，기록기수축활동，동시주CaCl2적량효곡선。결과염료목소능강저위장평활기조적장력，감소기수축파평균진폭。분타랍명、좌선-NG-초기정안산、격렬본뇨、SQ22536가부분조단염료목소대대서리체위장수축활동적억제작용。1．00 mol/L적염료목소대CaCl2적량효곡선무영향，50．0 mol/L염료목소사 CaCl2적량효곡선우이。결론염료목소대대서위장평활기조수축활동구유억제작용。
Objective To investigate the effects of genistein(Gen)on contractile activity of gastrointestinal smooth muscle of rat and explore its underlying mechanisms.Methods Gastrointestinal smooth muscle strips were taken out from rat.Each isolated muscle strip was put in a tissue chamber which contains 5 mL krebs solution and the motility of muscle strips was recorded simulta-neously.At the same time the CaCl2 concentration-response curves were done.Results Genistein reduced the resting tension of gas-trointestinal smooth muscle strips and decreased mean contractile amplitude of gastrointestinal smooth muscle strips,which were dose-dependent.Phentolamine,l-NG-nitroarginine,glibenclamide,SQ22536 partially blocked the inhibition of puerarin on the con-tractile activity of the rat gastric body.Genistein(1.00μmol/L)had no significant effect on calcium-dependent contraction,while genistein(50.00μmol/L)markedly prevented contractile effects of CaCl2 on body of stomach.Conclusion Genistein has inhibitory action on the isolated gastrointestinal smooth muscle strips of rats.