CAJ | 학술논문

目的：观察健脾化湿颗粒对腹泻型肠易激综合征（IBS‐D ）大鼠结肠黏膜5‐羟色胺（5‐H T ）表达的影响。方法60只清洁级Wistar大鼠，分为6组，每组10只。采用番泻叶灌胃配合束缚应激造模。造模成功后，健脾化湿颗粒低、中、高剂量组给药浓度分别为1．5、2．5、4．0 g/mL（生药），得舒特组给药浓度3 mg/mL灌胃给药。每日1次，连续2周，另外两组为不经任何处理的正常组和造模成功的模型组。实验结束免疫组织化学观察结肠标本5‐H T水平的变化。结果 IBS‐D模型大鼠结肠组织5‐HT的水平显著高于正常组（P＜0．01）；除健脾化湿低剂量组外，各治疗组大鼠结肠组织中5‐HT 的水平显著低于模型组（P＜0．01）。其中，健脾化湿颗粒高剂量组效果明显，与得舒特组差异无统计学意义（ P＞0．05）。结论健脾化湿颗粒可抑制IBS‐D模型大鼠结肠黏膜5‐H T过高表达，明显改善IB S‐D模型大鼠胃肠功能。
목적：관찰건비화습과립대복사형장역격종합정（IBS‐D ）대서결장점막5‐간색알（5‐H T ）표체적영향。방법60지청길급Wistar대서，분위6조，매조10지。채용번사협관위배합속박응격조모。조모성공후，건비화습과립저、중、고제량조급약농도분별위1．5、2．5、4．0 g/mL（생약），득서특조급약농도3 mg/mL관위급약。매일1차，련속2주，령외량조위불경임하처리적정상조화조모성공적모형조。실험결속면역조직화학관찰결장표본5‐H T수평적변화。결과 IBS‐D모형대서결장조직5‐HT적수평현저고우정상조（P＜0．01）；제건비화습저제량조외，각치료조대서결장조직중5‐HT 적수평현저저우모형조（P＜0．01）。기중，건비화습과립고제량조효과명현，여득서특조차이무통계학의의（ P＞0．05）。결론건비화습과립가억제IBS‐D모형대서결장점막5‐H T과고표체，명현개선IB S‐D모형대서위장공능。
Objective To observe the effect of Jian Pi Hua Shi Ke Li(JPHSKL) on IBS‐D ,gastrointestinal peptide hormones of rats 5‐HT ,and explore the therapeutic mechanism .Methods 60 clean‐level Wistar rats were used in the experiment .The rats were randomly divided into normal group ,model group ,JPHS low ,middle ,high and Deshute group(10 rats in each group) .Used gavage senna and restraint stress to establish D‐IBS rat model .The drug contrations of JPHSKL low ,midle ,high group were 1 .5 ,2 .5 ,4 .0 g/mL ,3 mg/mol in Deshute group .Once each day ,for 2 weeks ,the other groups were nomol group without any treatment and mod‐el group which building successful .Immunohistochemical SABC method to observe 5‐HT level of colonic specimens .Results The 5‐HT level in colonic tissue of the rats in IBS‐D model were obviously higher than the nomal group(P<0 .01) ,and the 5‐HT level in colonic tissue in JPHSKL treated group were obviously lower than the mold group (P<0 .05) .Among them ,the JPHSKL high group had significant effect and there was no obviously difference with Deshute group (P>0 .05) .Conclusion JPHSKL can im‐prove gastrointestinal function of IBS‐D model rats ,effect mechanism is relating to decrease the level of 5‐HT in colonic mucosa .