CAJ | 학술논문

目的：探讨双歧杆菌四联活菌片（以下简称四联活菌片）联合芪倍合剂治疗溃疡性结肠炎的临床疗效及对免疫系统的调节作用。方法应用流式细胞仪和ELISA法检测四联活菌片联合芪倍合剂治疗溃疡性结肠炎前后 T细胞亚群及外周血淋巴细胞Fas及FasL的表达水平，并与四联活菌片及芪倍合剂单一治疗进行比较。结果四联活菌片联合芪倍合剂治疗（第一组）总有效率为92．50％，高于四联活菌片单独治疗（第二组）（72．50％）（ P＜0．01）。各组治疗前外周血 T细胞（CD3＋）、辅助性T细胞（CD3＋CD4＋）、抑制性T细胞（CD3＋CD8＋）均高于健康对照组（ P＜0．01），CD4＋/CD8＋值升高（ P＜0．05），Fas表达明显升高（P＜0．05）。治疗后CD3＋、CD3＋ CD4＋、CD3＋ CD8＋较治疗前下降（P＜0．05），CD4＋/CD8＋值降低（P＜0．05），Fas明显下降（P＜0．01）。结论四联活菌片可通过逆转外周血淋巴细胞的Fas/FasL系统表达异常，诱导淋巴细胞的正常凋亡，与中药芪倍合剂合用起协同作用。
목적：탐토쌍기간균사련활균편（이하간칭사련활균편）연합기배합제치료궤양성결장염적림상료효급대면역계통적조절작용。방법응용류식세포의화ELISA법검측사련활균편연합기배합제치료궤양성결장염전후 T세포아군급외주혈림파세포Fas급FasL적표체수평，병여사련활균편급기배합제단일치료진행비교。결과사련활균편연합기배합제치료（제일조）총유효솔위92．50％，고우사련활균편단독치료（제이조）（72．50％）（ P＜0．01）。각조치료전외주혈 T세포（CD3＋）、보조성T세포（CD3＋CD4＋）、억제성T세포（CD3＋CD8＋）균고우건강대조조（ P＜0．01），CD4＋/CD8＋치승고（ P＜0．05），Fas표체명현승고（P＜0．05）。치료후CD3＋、CD3＋ CD4＋、CD3＋ CD8＋교치료전하강（P＜0．05），CD4＋/CD8＋치강저（P＜0．05），Fas명현하강（P＜0．01）。결론사련활균편가통과역전외주혈림파세포적Fas/FasL계통표체이상，유도림파세포적정상조망，여중약기배합제합용기협동작용。
Objective To estimate the clinical efficacy and immunomodulatory effect of tetralogy of viable tablets combined with Qibei mixture .Methods Flow aftometry and enzyme linked immunosorbent assay (ELISA) were performed to detect T cell subsets and expression level of Fas and FasL respectively .Results The response rate was significantly higher in patients treated with tet‐ralogy of viable tablets combined with Qibei mixture than in those with tetralogy of viable tablets alone (92 .50% vs .72 .50% ,P<0 .01) ,before the treatment ,patients showed a higher percentage of CD3+ cells ,CD3+CD4+ cells and CD3+CD8+ cells in peripheral blood(P<0 .01) ,weaker expression of Fas(P<0 .01) ,but a higher CD4+ /CD8+ ratio(P<0 .01)compared with the controls .The treatment with Qibei mixture and tetralogy of viable tablets alone or in combination all induced an decrease in the percentage of CD3+cells ,CD3+CD4+cells and CD3+CD8+ cells in peripheral blood(P<0 .05)and in the expression of Fas(P<0 .01) ,but an de‐crease in CD4+ /CD8+ ratio(P<0 .05) .Conclusion Tetralogy of viable tablets may induce the normal apoptosis in lymphocytes via reversing the abnomality in the expression of Fas/FasL by peripheral blood lymphocytes ,and the effect of tetralogy of viable tablets may be enhanced by Qibei mixture .