CAJ | 학술논문

目的：探讨育真熄风汤（YZXFT ）对小鼠帕金森病（PD）模型中脑铁蛋白Fn的影响。方法将试验动物分为空白对照组、模型对照组、阳性药物对照组和中药治疗组，在1‐甲基‐4‐苯基‐1，20，3，6‐四氢吡啶（MPTP）诱导的PD小鼠模型基础上，采用免疫组织化学染色，观察各组小鼠脑内Fn蛋白的表达情况。结果爬杆实验结果显示，中药治疗组小鼠与模型对照组小鼠比较，运动协调障碍有所减轻；免疫组织化学结果显示，第6、13、20天模型对照组 Fn水平与空白对照组比较，差异有统计学意义（ P＜0．05），分别为2．21倍、1．15倍、0．36倍。YZXFT 71．5 g/kg和143 g/kg组与模型对照组比较能明显增加Fn蛋白水平。结论 YZXFT具有提高脑内Fn蛋白表达作用，为进一步研究其治疗PD的机制提供依据。
목적：탐토육진식풍탕（YZXFT ）대소서파금삼병（PD）모형중뇌철단백Fn적영향。방법장시험동물분위공백대조조、모형대조조、양성약물대조조화중약치료조，재1‐갑기‐4‐분기‐1，20，3，6‐사경필정（MPTP）유도적PD소서모형기출상，채용면역조직화학염색，관찰각조소서뇌내Fn단백적표체정황。결과파간실험결과현시，중약치료조소서여모형대조조소서비교，운동협조장애유소감경；면역조직화학결과현시，제6、13、20천모형대조조 Fn수평여공백대조조비교，차이유통계학의의（ P＜0．05），분별위2．21배、1．15배、0．36배。YZXFT 71．5 g/kg화143 g/kg조여모형대조조비교능명현증가Fn단백수평。결론 YZXFT구유제고뇌내Fn단백표체작용，위진일보연구기치료PD적궤제제공의거。
Objective To study the effect of Yuzhenxifeng Decoction on the brain ferritin in PD mouse models .Methods All ex‐perimental animals were devided into 4 groups:the control group ,the model group ,the positive drug group and TCM group .Prepare PD model mice with MPTP ,then use the immunohistochemical staining technique to observe the change of expression of Fn .Results the results showed that compared with the model group ,movement coordination disorder of rats in TCM group were relieved ;the Fn level of the model group on 6th ,13th ,20th were higher than control group(P<0 .05) ,and were 2 .21 times ,1 .15 times and 0 .36 times higher respectively .Compared with model group mice ,expression of Fn were enhanced in the treatment group .Conclusion Yuzhenxifeng Decoction can improve the expression of Fn in the brain ,which provide the basis for further study on mechanism of the treatment of Parkinson′s disease .