CAJ | 학술논문

目的：探讨NO电化学微传感器在抗细菌感染免疫研究中的应用价值。方法应用前期制备的基于纳米金（nano‐Au）修饰玻璃纤维的新型NO电化学微传感器实时检测大肠埃希菌（E ．coli）不同浓度刺激组、不同时间刺激组的小鼠巨噬细胞（RAW264．7细胞）NO的释放水平。结果与对照组相比，RAW264．7细胞受到E ．coli刺激后，NO的释放水平明显上调（ P＜0．01），且对E ．coli的刺激具有浓度依耐性。随着E ．coli作用时间的延续，RAW264．7细胞的 NO释放水平逐渐上升，作用12 h时达到高峰，然后开始下降。结论 NO电化学微传感器成功应用于E ．coli激活的巨噬细胞释放NO过程的实时检测。
목적：탐토NO전화학미전감기재항세균감염면역연구중적응용개치。방법응용전기제비적기우납미금（nano‐Au）수식파리섬유적신형NO전화학미전감기실시검측대장애희균（E ．coli）불동농도자격조、불동시간자격조적소서거서세포（RAW264．7세포）NO적석방수평。결과여대조조상비，RAW264．7세포수도E ．coli자격후，NO적석방수평명현상조（ P＜0．01），차대E ．coli적자격구유농도의내성。수착E ．coli작용시간적연속，RAW264．7세포적 NO석방수평축점상승，작용12 h시체도고봉，연후개시하강。결론 NO전화학미전감기성공응용우E ．coli격활적거서세포석방NO과정적실시검측。
Objective To apply nitric oxide(NO) electrochemical microsensor in the real time detection of NO released from RAW 264 .3 cells infected by E .coli ,and to explore the application value of this NO microsensor in the research area of infection im‐munity against bacterium .Methods Taking NO microsensor to detect NO released from RAW 264 .3 cells respectively stimulated by E .coli of different densities and of 1 × 107 mL -1 for different time .Results The level of NO released from RAW 264 .3 cells was enhanced obviously when incubated with E .coli as compared with that of normal cells and the extent of incersase depended on the density of E .coli (P<0 .01) .The released level of NO increased gradually from the beginning and reached its peal at the time of 12 h then decreased slowly when incubated with E .coli of 1 × 107 mL -1 .Conclusion The electrochemical microsensor was applied in the real time detection of NO released from macrophages activated by E .coli successfully .