微生物与感染
微生物與感染
미생물여감염
JOURNAL OF MICROBES AND INFECTION
2014年
2期
112-115
,共4页
张硕%李建东%曲靖%张福顺%李川%梁米芳%李德新
張碩%李建東%麯靖%張福順%李川%樑米芳%李德新
장석%리건동%곡정%장복순%리천%량미방%리덕신
发热伴血小板减少综合征病毒%树突细胞特异性细胞间黏附分子3结合非整合素因子%受体
髮熱伴血小闆減少綜閤徵病毒%樹突細胞特異性細胞間黏附分子3結閤非整閤素因子%受體
발열반혈소판감소종합정병독%수돌세포특이성세포간점부분자3결합비정합소인자%수체
Severe fever with thrombocytopenia syndrome virus%Dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin%Receptor
为初步研究树突细胞特异性细胞间黏附分子3结合非整合素因子(DC-SIGN)是否能作为发热伴血小板减少综合征病毒(SFTSV)的细胞表面受体,首先通过实时反转录-聚合酶链反应(RT-PCR)检测不同细胞系DC-SIGN mRNA水平及SFTSV感染水平,并用DC-SIGN特异性单克隆抗体封闭SFTSV敏感细胞,研究其对SFTSV感染水平的影响。此外,将DC-SIGN表达载体转染不同细胞系,研究转染前后细胞中SFTSV感染水平的变化。结果显示,在SFTSV感染水平较高的 Vero细胞中,DC-SIGN mRNA水平较高;而在SFTSV感染水平低于Vero细胞的CHO-K1细胞中,DC-SIGN mRNA水平较低。DC-SIGN单克隆抗体在一定程度上能抑制病毒进入宿主细胞,并呈现剂量-效应关系,DC-SIGN可提高 Vero和 CHO-K1细胞中SFTSV的感染水平。本研究表明DC-SIGN为SFTSV的可能受体。
為初步研究樹突細胞特異性細胞間黏附分子3結閤非整閤素因子(DC-SIGN)是否能作為髮熱伴血小闆減少綜閤徵病毒(SFTSV)的細胞錶麵受體,首先通過實時反轉錄-聚閤酶鏈反應(RT-PCR)檢測不同細胞繫DC-SIGN mRNA水平及SFTSV感染水平,併用DC-SIGN特異性單剋隆抗體封閉SFTSV敏感細胞,研究其對SFTSV感染水平的影響。此外,將DC-SIGN錶達載體轉染不同細胞繫,研究轉染前後細胞中SFTSV感染水平的變化。結果顯示,在SFTSV感染水平較高的 Vero細胞中,DC-SIGN mRNA水平較高;而在SFTSV感染水平低于Vero細胞的CHO-K1細胞中,DC-SIGN mRNA水平較低。DC-SIGN單剋隆抗體在一定程度上能抑製病毒進入宿主細胞,併呈現劑量-效應關繫,DC-SIGN可提高 Vero和 CHO-K1細胞中SFTSV的感染水平。本研究錶明DC-SIGN為SFTSV的可能受體。
위초보연구수돌세포특이성세포간점부분자3결합비정합소인자(DC-SIGN)시부능작위발열반혈소판감소종합정병독(SFTSV)적세포표면수체,수선통과실시반전록-취합매련반응(RT-PCR)검측불동세포계DC-SIGN mRNA수평급SFTSV감염수평,병용DC-SIGN특이성단극륭항체봉폐SFTSV민감세포,연구기대SFTSV감염수평적영향。차외,장DC-SIGN표체재체전염불동세포계,연구전염전후세포중SFTSV감염수평적변화。결과현시,재SFTSV감염수평교고적 Vero세포중,DC-SIGN mRNA수평교고;이재SFTSV감염수평저우Vero세포적CHO-K1세포중,DC-SIGN mRNA수평교저。DC-SIGN단극륭항체재일정정도상능억제병독진입숙주세포,병정현제량-효응관계,DC-SIGN가제고 Vero화 CHO-K1세포중SFTSV적감염수평。본연구표명DC-SIGN위SFTSV적가능수체。
To study cell surface receptors of severe fever with thrombocytopenia syndrome virus (SFTSV ) , the levels of dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN ) mRNA and SFTSV infection in different cell lines were identified by real-time reverse transcriptase-polymerase chain reaction (RT-PCR ) .SFTSV-sensitive cells were inhibited by anti-DC-SIGN monoclonal antibody (mAb) and virus infection levels were detected by real-time RT-PCR .Different cell lines were transfected with DC-SIGN-expressing plasmid and SFTSV infection levels either before or after transfection were detected .The results showed that Vero cells which were sensitive to SFTSV had a relatively higher DC-SIGN mRNA level than CHO-K1 cells which were less sensitive to SFTSV .Anti-DC-SIGN mAb was capable to inhibit SFTSV invasion into host cells to a certain extent ,showing a dose-response effect .The levels of SFTSV infection in cells transfected with DC-SIGN-expressing plasmid were higher than those in non-transfected cells .It is suggested that DC-SIGN is probable one of the cell surface receptors of SFTSV .