健康研究
健康研究
건강연구
HEALTH RESEARCH
2014年
3期
251-253
,共3页
雷奈酸锶%失用性骨质疏松%骨代谢%骨密度%骨组织形态计量学%生物力学
雷奈痠鍶%失用性骨質疏鬆%骨代謝%骨密度%骨組織形態計量學%生物力學
뢰내산송%실용성골질소송%골대사%골밀도%골조직형태계량학%생물역학
strontium ranelate%disuse-induced osteoporosis%bone metablism%bone mineral density%bone histomorphometry%biomechanics
目的：通过干预拟失重大鼠骨质疏松动物模型，探讨雷奈酸锶对拟失重大鼠骨量、骨代谢和生物力学性能的影响。方法3月龄SD大鼠24只，随机均分为对照组（A）、骨质疏松组（B）、雷奈酸锶组（C），每组8只。 B、C两组大鼠采用尾悬吊法制备失用性骨质疏松大鼠模型，C组给予雷奈酸锶干预（1g/kg/d）。4周后处死所有大鼠，留取尿液、血清检测血P1NP值、尿DPYD/Cr、NTX/Cr值，右侧胫骨制备非脱钙组织切片并行骨形态计量学检测，右侧股骨检测骨密度，取左侧股骨行三点弯曲试验，检测其最大载荷。结果 B组血P1NP值显著低于A、C组，尿DPYD/Cr、尿NTX/Cr值显著高于A、C组，C组显著高于A组（均P＜0．05）；B组骨小梁体积、骨小梁数量、骨小梁厚度、骨形成率显著低于A、C组，B组破骨细胞数、骨吸收长度比显著高于A、C组；股骨骨密度由高到低依次为A、C、B组，组间差异均有显著性（P＜0．05）；最大载荷B组显著低于A组（P＜0．05）。结论雷奈酸锶可部分阻止尾悬吊导致的大鼠骨质量和力学性能的下降。
目的：通過榦預擬失重大鼠骨質疏鬆動物模型，探討雷奈痠鍶對擬失重大鼠骨量、骨代謝和生物力學性能的影響。方法3月齡SD大鼠24隻，隨機均分為對照組（A）、骨質疏鬆組（B）、雷奈痠鍶組（C），每組8隻。 B、C兩組大鼠採用尾懸弔法製備失用性骨質疏鬆大鼠模型，C組給予雷奈痠鍶榦預（1g/kg/d）。4週後處死所有大鼠，留取尿液、血清檢測血P1NP值、尿DPYD/Cr、NTX/Cr值，右側脛骨製備非脫鈣組織切片併行骨形態計量學檢測，右側股骨檢測骨密度，取左側股骨行三點彎麯試驗，檢測其最大載荷。結果 B組血P1NP值顯著低于A、C組，尿DPYD/Cr、尿NTX/Cr值顯著高于A、C組，C組顯著高于A組（均P＜0．05）；B組骨小樑體積、骨小樑數量、骨小樑厚度、骨形成率顯著低于A、C組，B組破骨細胞數、骨吸收長度比顯著高于A、C組；股骨骨密度由高到低依次為A、C、B組，組間差異均有顯著性（P＜0．05）；最大載荷B組顯著低于A組（P＜0．05）。結論雷奈痠鍶可部分阻止尾懸弔導緻的大鼠骨質量和力學性能的下降。
목적：통과간예의실중대서골질소송동물모형，탐토뢰내산송대의실중대서골량、골대사화생물역학성능적영향。방법3월령SD대서24지，수궤균분위대조조（A）、골질소송조（B）、뢰내산송조（C），매조8지。 B、C량조대서채용미현조법제비실용성골질소송대서모형，C조급여뢰내산송간예（1g/kg/d）。4주후처사소유대서，류취뇨액、혈청검측혈P1NP치、뇨DPYD/Cr、NTX/Cr치，우측경골제비비탈개조직절편병행골형태계량학검측，우측고골검측골밀도，취좌측고골행삼점만곡시험，검측기최대재하。결과 B조혈P1NP치현저저우A、C조，뇨DPYD/Cr、뇨NTX/Cr치현저고우A、C조，C조현저고우A조（균P＜0．05）；B조골소량체적、골소량수량、골소량후도、골형성솔현저저우A、C조，B조파골세포수、골흡수장도비현저고우A、C조；고골골밀도유고도저의차위A、C、B조，조간차이균유현저성（P＜0．05）；최대재하B조현저저우A조（P＜0．05）。결론뢰내산송가부분조지미현조도치적대서골질량화역학성능적하강。
Objective To understand the effects of strontium ranelate on bone mass , bone matablism and biomechanical properties of osteoporotic rats induced by disuse .Methods 24 3-month old SD rats were randomized into 3 groups of 8 animals each:Normal control group ( A) , osteoporosis group ( B) , and strontium ranelate treatment group ( C) .Rats in group B and C were subjected to tail suspension , and rats in group C were administered with strontium ranelate (1g/kg/d).All rats were sacrificed 4 weeks later .Content of P1NP in blood serum and DPYD/Cr, NTX/Cr in urine were detected .The right tibias were prepared for undecalcified sections and thereby bone histomorphometry analysis , and right femurs were collected for bone mineral density assessment , three-point bending test was performed on left femurs to assess the maximum load . Results 1.P1NP concentration in group B was significantly lower than that of group A and C .Tail suspension markedly increased the levels of DPYD/Cr and NTX/Cr.Strontium ranelate could retard the increase but still significantly higher than those of normal rats (P <0.05); 2.Trabecular volume, number, thickness, and bone formation rate in group B were significantly lower than those in group A and C , while the osteoclast number and erosion percentage were markedly higher in group B, as compared to those of group A and C (P<0.05);3.Bone mineral density in group A, C and B showed a subsequently decreasing manner , with significant difference between any two groups ( P <0.05 ); 4.The results of biomechanical test showed markedly lower maximum load in group B as compared to group A (P<0.05).Conclusion Strontium ranelate could partially prevent the deterioration of bone quality and biomechanical property in disuse -induced osteoporosis rat .
