海洋科学
海洋科學
해양과학
MARINE SCIENCES
2014年
3期
1-5
,共5页
王鸣慧%李晓明%李春顺%王斌贵
王鳴慧%李曉明%李春順%王斌貴
왕명혜%리효명%리춘순%왕빈귀
沉积物%嗜松青霉(Penicillium pinophilum)%次生代谢产物%卤虫致死活性
沉積物%嗜鬆青黴(Penicillium pinophilum)%次生代謝產物%滷蟲緻死活性
침적물%기송청매(Penicillium pinophilum)%차생대사산물%서충치사활성
sediment%Penicillium pinophilum%secondary metabolite%brine shrimp lethality
对来源于珠江口沉积物的一株海洋真菌嗜松青霉(Penicillium pinophilum)SD-272的次生代谢产物,进行了化学成分分离及其生物活性研究。采用常规硅胶柱层析、凝胶Sephadex LH-20柱层析、制备薄层层析等分离手段,通过紫外、核磁共振技术、质谱技术等现代波谱学技术,从其发酵液提取物中共分离并鉴定了13个化合物,分别为：4￠-demethylvermistatin (1), vermistatin (2), penisimplicis-sin(3), deoxyfunicone (4),5,6-epoxy-3-deoxyfunicone (5),5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (6), altenusin (7),1-deoxyrubralactone(8), kojic acid (9),7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (10), dankasterone (11),4-hydroxy-2-methoxyacetanilide (12), N-(2-hydroxypropanoyl)-2-aminobenzoic acid amide (13)。这些化合物均为首次从嗜松青霉中分离得到,其中化合物11显示较好的卤虫致死活性, LD50值为39.2μmol/L。
對來源于珠江口沉積物的一株海洋真菌嗜鬆青黴(Penicillium pinophilum)SD-272的次生代謝產物,進行瞭化學成分分離及其生物活性研究。採用常規硅膠柱層析、凝膠Sephadex LH-20柱層析、製備薄層層析等分離手段,通過紫外、覈磁共振技術、質譜技術等現代波譜學技術,從其髮酵液提取物中共分離併鑒定瞭13箇化閤物,分彆為：4￠-demethylvermistatin (1), vermistatin (2), penisimplicis-sin(3), deoxyfunicone (4),5,6-epoxy-3-deoxyfunicone (5),5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (6), altenusin (7),1-deoxyrubralactone(8), kojic acid (9),7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (10), dankasterone (11),4-hydroxy-2-methoxyacetanilide (12), N-(2-hydroxypropanoyl)-2-aminobenzoic acid amide (13)。這些化閤物均為首次從嗜鬆青黴中分離得到,其中化閤物11顯示較好的滷蟲緻死活性, LD50值為39.2μmol/L。
대래원우주강구침적물적일주해양진균기송청매(Penicillium pinophilum)SD-272적차생대사산물,진행료화학성분분리급기생물활성연구。채용상규규효주층석、응효Sephadex LH-20주층석、제비박층층석등분리수단,통과자외、핵자공진기술、질보기술등현대파보학기술,종기발효액제취물중공분리병감정료13개화합물,분별위：4￠-demethylvermistatin (1), vermistatin (2), penisimplicis-sin(3), deoxyfunicone (4),5,6-epoxy-3-deoxyfunicone (5),5′-methoxy-6-methyl-biphenyl-3,4,3′-triol (6), altenusin (7),1-deoxyrubralactone(8), kojic acid (9),7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (10), dankasterone (11),4-hydroxy-2-methoxyacetanilide (12), N-(2-hydroxypropanoyl)-2-aminobenzoic acid amide (13)。저사화합물균위수차종기송청매중분리득도,기중화합물11현시교호적서충치사활성, LD50치위39.2μmol/L。
In this paper, thirteen compounds were isolated from the zymotic fluid extract of the fungal strain Penicillium pinophilum SD-272by a combination of silica gel, Sephadex LH-20, and Lobar LiChroprep RP-18 column chromatography as well as the preparative thin layer chromatography. SD-272was isolated from sediment sample collected from the estuary of the Pearl River in South China Sea. The structures of these compounds were elucidated mainly based on the analysis of the UV, MS, 1D and 2D NMR as 4￠-demethylvermistatin (1), vermistatin (2), penisimplicissin(3), deoxyfunicone (4), 5, 6-epoxy-3-deoxyfunicone (5), 5￠-methoxy-6-methyl-biphenyl-3, 4, 3′-triol (6), altenusin (7), 1-deoxyrubralactone(8), kojic acid (9), 7-hydroxy-2-(2-hydroxypropyl)-5-methylchromone (10), dankasterone (11), 4-hydroxy-2-methoxyacetanilide (12), and N-(2-hydroxypropanoyl)-2-aminobenzoic acid amide (13). All the compounds were firstly reported to be isolated from P.pinophilum. Compound 11 displayed potent brine shrimp lethality with a LD50 of 39.2μmol/L.
