CAJ | 학술논문

在模拟人体生理条件下，应用荧光和紫外-可见光谱法研究了糖精钠与牛血清白蛋白（BS A ）之间的相互作用；并计算了不同温度下的热力学参数、结合常数和结合位点数。结果表明，糖精钠对BSA的猝灭机制属于形成复合物的静态猝灭过程；两者之间的作用主要是氢键或范德华力，作用的位点更靠近色氨酸；金属离子对两者的作用具有一定的影响。
재모의인체생리조건하，응용형광화자외-가견광보법연구료당정납여우혈청백단백（BS A ）지간적상호작용；병계산료불동온도하적열역학삼수、결합상수화결합위점수。결과표명，당정납대BSA적졸멸궤제속우형성복합물적정태졸멸과정；량자지간적작용주요시경건혹범덕화력，작용적위점경고근색안산；금속리자대량자적작용구유일정적영향。
The interaction of saccharin sodium with bovine serum albumin (BSA) under simula-ted physiological conditions was investigated by fluorescence spectrometry and ultraviolet-visi-ble light absorption spectrometry ,and the binding constants ,number of binding sites and ther-modynamic parameters under different temperature were calculated .Results show that the quenching of BSA by saccharin sodium is a static quenching procedure involving complex for-mation .The interaction between BSA and saccharin sodium is dominated by Van der Waals forces or hydrogen bond ,and the binding site of saccharin sodium and BSA is close to trypto-phan residue .In addition ,metal ions influence the interaction of saccharin sodium and BSA to some extent .