CAJ | 학술논문

目的：探讨PCR方法在分析血流感染病原菌分布中的作用，为儿童细菌性血流感染的流行病学调查提供新的思路。方法从儿科重症监护病房（PICU）收集100份疑似血流感染患儿的外周静脉血进行血培养，同时扩增并测序细菌16S rDNA，比较培养结果与PCR结果。结果100份血液标本中11份细菌培养阳性，阳性率为11%；PCR法23份阳性，阳性率为23%，二者比较差异具有统计学意义(χ2=10.08，P<0.05)；在23例PCR阳性标本中，革兰氏阳性球菌占65.2%，其中表皮葡萄球菌最多(6例，26.1%)，革兰氏阴性菌占34.8%，以大肠埃希菌为主(4例，17.4%)。结论16S rDNA-PCR结合测序的方法可以很好地鉴定血流感染病原菌，与血培养相比能更客观地反映病原菌分布，可作为一种新的流行病学方法在临床应用；儿科重症监护病房血流感染病原菌以革兰氏阳性球菌为主，尤以表皮葡萄球菌最常见。
목적：탐토PCR방법재분석혈류감염병원균분포중적작용，위인동세균성혈류감염적류행병학조사제공신적사로。방법종인과중증감호병방（PICU）수집100빈의사혈류감염환인적외주정맥혈진행혈배양，동시확증병측서세균16S rDNA，비교배양결과여PCR결과。결과100빈혈액표본중11빈세균배양양성，양성솔위11%；PCR법23빈양성，양성솔위23%，이자비교차이구유통계학의의(χ2=10.08，P<0.05)；재23례PCR양성표본중，혁란씨양성구균점65.2%，기중표피포도구균최다(6례，26.1%)，혁란씨음성균점34.8%，이대장애희균위주(4례，17.4%)。결론16S rDNA-PCR결합측서적방법가이흔호지감정혈류감염병원균，여혈배양상비능경객관지반영병원균분포，가작위일충신적류행병학방법재림상응용；인과중증감호병방혈류감염병원균이혁란씨양성구균위주，우이표피포도구균최상견。
Objective To explore the value of PCR for analyzing the pathogenic spectrum of bloodstream in-fection (BSI) in children, and to provide a new thought for epidemiological survey of BSI. Methods A total of 100 patients in PICU with suspected bacterial infections were included, and then performed blood cultivation (BC) and an-alyzed by 16S rDNA-PCR following by sequencing and BLAST. At the same time, the results of two methods were compared. Results Among the 100 specimens, the positive rate of BC and PCR are 11% and 23%, respectively. There was significant difference between the two methods (χ2=10.08, P<0.05). Gram-positive bacteria and Gram-nega-tive bacteria accounted for 65.2%and 34.8%, respectively, in 23 bacteria. The predominant pathogens were Staphylo-coccus epidermidis (26.1%), followed by Escherichia coil (17.4%). Conclusions Compared with BC, 16S rD-NA-PCR and sequencing which provide a new way of analyzing the pathogenic spectrum of BSI might be effective for epidemiological investigation. The pathogens of BSI are mainly gram-positive bacteria in PICU, and Staphylococ-cus epidermidis is the most common one.