CAJ | 학술논문

目的：观察酸枣仁汤对快动眼（ REM）睡眠剥夺导致的老年大鼠脑皮层和海马部位神经细胞凋亡及c-fos基因表达Fos蛋白水平变化的影响。方法将自然衰老24月龄Wistar大鼠随机分成空白对照组（等容生理盐水），老年REM睡眠剥夺组（等容生理盐水），阳性对照组（舒乐安定0.18 mg／（kg· d）），酸枣仁汤低、高剂量组（12.96，25.92 g／（ kg· d））。各组灌胃给药2周后，除空白对照组外其余各组用自制改良多平台法剥夺大鼠睡眠48 h制作老年大鼠REM睡眠剥夺模型。通过原位末端标记法（ TUNEL）测定各组大鼠大脑皮层和海马部位神经细胞凋亡水平，用免疫组化法检测c-fos基因表达Fos蛋白的水平变化，探讨酸枣仁汤对老年失眠引起的脑神经损害及c-fos基因表达变化的影响。结果与空白对照组比较，老年REM睡眠剥夺组大鼠脑皮层和海马部位神经凋亡细胞增多， Fos蛋白表达明显增高。与老年REM睡眠剥夺组比较，酸枣仁汤组脑神经凋亡细胞及Fos蛋白表达明显下降，但仍高于正常对照组。结论c-fos基因很可能参与了老年大鼠REM睡眠剥夺后的细胞凋亡过程，酸枣仁汤可能是通过抑制脑组织c-fos基因表达上调，抑制神经细胞凋亡而发挥脑保护作用。
목적：관찰산조인탕대쾌동안（ REM）수면박탈도치적노년대서뇌피층화해마부위신경세포조망급c-fos기인표체Fos단백수평변화적영향。방법장자연쇠로24월령Wistar대서수궤분성공백대조조（등용생리염수），노년REM수면박탈조（등용생리염수），양성대조조（서악안정0.18 mg／（kg· d）），산조인탕저、고제량조（12.96，25.92 g／（ kg· d））。각조관위급약2주후，제공백대조조외기여각조용자제개량다평태법박탈대서수면48 h제작노년대서REM수면박탈모형。통과원위말단표기법（ TUNEL）측정각조대서대뇌피층화해마부위신경세포조망수평，용면역조화법검측c-fos기인표체Fos단백적수평변화，탐토산조인탕대노년실면인기적뇌신경손해급c-fos기인표체변화적영향。결과여공백대조조비교，노년REM수면박탈조대서뇌피층화해마부위신경조망세포증다， Fos단백표체명현증고。여노년REM수면박탈조비교，산조인탕조뇌신경조망세포급Fos단백표체명현하강，단잉고우정상대조조。결론c-fos기인흔가능삼여료노년대서REM수면박탈후적세포조망과정，산조인탕가능시통과억제뇌조직c-fos기인표체상조，억제신경세포조망이발휘뇌보호작용。
Objective To observe the effect of Suanzao-Rentang on neuronal apoptosis and c-fos gene expression in Fos protein levels in cortex and hippocampus of aged rats with rapid eye movement ( REM) sleep deprivation.Methods Natu-ral aging 24 months old Wistar rats were randomly divided into control group (isovolumetric saline),REM sleep deprivation eld-erly group (isovolumetric saline),positive control group estazolam 0.18mg/(kg· d),Suanzao-Rentang low and high dose group 12.96,25.92 g/(kg· d).Each group was given intragastric administration for two weeks,then each group except the control group is made REM sleep deprivation for 48h to produce model of old rats with homemade improved multi-platform.The nerve cells apoptosis of cortex and hippocampus in the rats from each group are determined by situ end labeling ( TUNEL) and level changes in c-fos gene expression to Fos protein are detected by using immunohistochemistry and explore the effect of suanzao-Rentang on the nerve damage and c-fos gene expression change in elder,which caused by insomnia.Results Compared with the control group,the cerebral cortex and hippocampus nerve cells apoptosis of rats, which are from REM sleep deprivation group, increased, and their Fos protein expression was significantly.Compared with REM sleep deprivation older group,Suanzao-Rent-ang group’s nerve cells apoptosis and Fos protein expression in brain significantly decreased,but still higher than the normal con-trol group.Conclusion C-fos gene is likely involved in the process of apoptosis in old REM sleep deprivation rats and Suanzao-Rentang inhibit neuronal apoptosis and play a protective role in the brain possibly by inhibiting brain upregulation of c-fos gene.