暨南大学学报(自然科学与医学版)
暨南大學學報(自然科學與醫學版)
기남대학학보(자연과학여의학판)
JOURNAL OF JINAN UNIVERSITY(NATURAL SCIENCE & MEDICINE EDITION)
2014年
2期
161-166
,共6页
林介夫%钟曌%梁赵佳%汪梦霞%李健玲%胡冬华%李雅兰
林介伕%鐘曌%樑趙佳%汪夢霞%李健玲%鬍鼕華%李雅蘭
림개부%종조%량조가%왕몽하%리건령%호동화%리아란
1 型糖尿病%大鼠%心肌缺血再灌注%模型
1 型糖尿病%大鼠%心肌缺血再灌註%模型
1 형당뇨병%대서%심기결혈재관주%모형
T1 DM%rats%myocardial ischemia-reperfusion%model
目的:探讨在1型糖尿病(T1 DM)基础上如何有效建立大鼠在体心肌缺血再灌注(IR)模型.方法:SPF级SD雄性大鼠72只,随机分为6组(N1、N2、N3、T1、T2、T3组),N1、N2、N3组血糖正常,T1、T2、T3组实施尾静脉注射65 mg/kg剂量链脲佐菌素(STZ)T1DM造模,N1、T1组IR时间为30/90 min,N2、T2组IR时间为60/90 min, N3、T3组IR时间为60/180 min,每组12只.T1DM造模成功后,与正常血糖大鼠均饲养8 w,麻醉后开胸行心肌缺血再灌注,再灌注结束后经右颈静脉注入伊文思蓝染色,剪下大鼠心脏并洗净置于-80℃,冻结后应用心肌切片模具将心脏切成1 mm厚度薄片,置于含质量分数1%TTC与10%甲醛的磷酸盐缓冲液中染色固定,37℃下避光静置20 min,取出切片拭净残液,数码相机拍照,应用ImageJ图像处理软件计算心肌梗死面积.结果:N1、N2、N3、T1、T2、T3组心梗面积分别为(31.08±7.78)%、(39.31±11.89)%、(39.18±8.33)%、(32.16±9.57)%、(44.96±9.07)%、(45.99±12.10)%,N1组与N2、N3组之间差异均有统计学意义(P=0.042,P=0.046),T1组与T2、T3组之间差异均有统计学意义(P=0.008,P=0.010),N1组与T1组之间差异无统计学意义(P=0.785),N2组与T2组之间差异有统计学意义(P<0.05),N3组与T3组之间差异有统计学意义(P<0.05).结论:大鼠在体IR中缺血60 min模型的心梗面积显著大于缺血30 min模型,在T1 DM状态下,最佳心肌缺血时间在30~60 min之间.
目的:探討在1型糖尿病(T1 DM)基礎上如何有效建立大鼠在體心肌缺血再灌註(IR)模型.方法:SPF級SD雄性大鼠72隻,隨機分為6組(N1、N2、N3、T1、T2、T3組),N1、N2、N3組血糖正常,T1、T2、T3組實施尾靜脈註射65 mg/kg劑量鏈脲佐菌素(STZ)T1DM造模,N1、T1組IR時間為30/90 min,N2、T2組IR時間為60/90 min, N3、T3組IR時間為60/180 min,每組12隻.T1DM造模成功後,與正常血糖大鼠均飼養8 w,痳醉後開胸行心肌缺血再灌註,再灌註結束後經右頸靜脈註入伊文思藍染色,剪下大鼠心髒併洗淨置于-80℃,凍結後應用心肌切片模具將心髒切成1 mm厚度薄片,置于含質量分數1%TTC與10%甲醛的燐痠鹽緩遲液中染色固定,37℃下避光靜置20 min,取齣切片拭淨殘液,數碼相機拍照,應用ImageJ圖像處理軟件計算心肌梗死麵積.結果:N1、N2、N3、T1、T2、T3組心梗麵積分彆為(31.08±7.78)%、(39.31±11.89)%、(39.18±8.33)%、(32.16±9.57)%、(44.96±9.07)%、(45.99±12.10)%,N1組與N2、N3組之間差異均有統計學意義(P=0.042,P=0.046),T1組與T2、T3組之間差異均有統計學意義(P=0.008,P=0.010),N1組與T1組之間差異無統計學意義(P=0.785),N2組與T2組之間差異有統計學意義(P<0.05),N3組與T3組之間差異有統計學意義(P<0.05).結論:大鼠在體IR中缺血60 min模型的心梗麵積顯著大于缺血30 min模型,在T1 DM狀態下,最佳心肌缺血時間在30~60 min之間.
목적:탐토재1형당뇨병(T1 DM)기출상여하유효건립대서재체심기결혈재관주(IR)모형.방법:SPF급SD웅성대서72지,수궤분위6조(N1、N2、N3、T1、T2、T3조),N1、N2、N3조혈당정상,T1、T2、T3조실시미정맥주사65 mg/kg제량련뇨좌균소(STZ)T1DM조모,N1、T1조IR시간위30/90 min,N2、T2조IR시간위60/90 min, N3、T3조IR시간위60/180 min,매조12지.T1DM조모성공후,여정상혈당대서균사양8 w,마취후개흉행심기결혈재관주,재관주결속후경우경정맥주입이문사람염색,전하대서심장병세정치우-80℃,동결후응용심기절편모구장심장절성1 mm후도박편,치우함질량분수1%TTC여10%갑철적린산염완충액중염색고정,37℃하피광정치20 min,취출절편식정잔액,수마상궤박조,응용ImageJ도상처리연건계산심기경사면적.결과:N1、N2、N3、T1、T2、T3조심경면적분별위(31.08±7.78)%、(39.31±11.89)%、(39.18±8.33)%、(32.16±9.57)%、(44.96±9.07)%、(45.99±12.10)%,N1조여N2、N3조지간차이균유통계학의의(P=0.042,P=0.046),T1조여T2、T3조지간차이균유통계학의의(P=0.008,P=0.010),N1조여T1조지간차이무통계학의의(P=0.785),N2조여T2조지간차이유통계학의의(P<0.05),N3조여T3조지간차이유통계학의의(P<0.05).결론:대서재체IR중결혈60 min모형적심경면적현저대우결혈30 min모형,재T1 DM상태하,최가심기결혈시간재30~60 min지간.
Aim:To investigate a reliable method of in vivo model of myocardial ischemia-reperfusion (IR)injury based on type 1 diabetes mellitus(T1 DM)in rats.Methods:Seventy-two male SD rats were randomly divided into 6 groups(N1,N2,N3,T1,T2,T3;n=12),of which 3 groups are with normal glu-cose levels(N1,N2,N3)and the other 3 are STZ-induced T1DMrats,dosed 65 mg/kg by caudal vein in-jection.All rats are respectively subjected to 30/90 min(N1,T1),60/90 min(N2,T2)and 60/180 min (N3,T3)IR duration.All rats were raised for 8 weeks after induction of T1DM.Myocardium was dyed by jugular vein injection of evans blue when IR model was made in vivo,then cut off and transfered to-80 ℃fridge.1 mm-thickness myocardial biopsy was made by a specific slice mould.Photographs were taken after slices were immobilized in 1%TTC and 10%methanal solution for 20 min in dark,then ana-lyzed with software to determine the infarct size.Results:The infarct size in groups N2 and groups N3 were stastically larger than in group N1 respectively(P=0.042,P=0.046),while the infarct size in group T2 and group T3 were also larger than in group T1(P=0.008,P=0.010).There is no significant difference in the infarct size between group N1 and group T1.Conclusion:Compared to 30 min-ischemia model,the infarct size in 60 min-ischemia model is signally larger for in vivo IR model of rats.The best ischemia duration of IR model is between 30 to 60 minutes In T1 DM rats.