中国烧伤创疡杂志
中國燒傷創瘍雜誌
중국소상창양잡지
2014年
3期
178-182
,共5页
李伙新%胡凤龙%曲心宇%欧阳昕%战玉军%张扬
李夥新%鬍鳳龍%麯心宇%歐暘昕%戰玉軍%張颺
리화신%호봉룡%곡심우%구양흔%전옥군%장양
烧伤%炎症%内毒素%外周血单个核细胞%肿瘤坏死因子-α%白细胞介素-1β%白细胞介素-6%白细胞介素-12
燒傷%炎癥%內毒素%外週血單箇覈細胞%腫瘤壞死因子-α%白細胞介素-1β%白細胞介素-6%白細胞介素-12
소상%염증%내독소%외주혈단개핵세포%종류배사인자-α%백세포개소-1β%백세포개소-6%백세포개소-12
Burns%Inflammation%Endotoxin%Peripheral blood mononuclear cells%Tumor necrosis factor-a%In-terleukin-1β%Interleukin-6%Interleukin-12
目的：探讨烧伤患者血清内毒素对正常机体外周血单个核细胞（ peripheral blood mononuclear cells， PBMCs）中4种炎性细胞因子的影响。方法以正常人外周血单个核细胞为受试对象，以烧伤患者血清为刺激因素。根据18例烧伤患者与6名健康志愿者血清内毒素水平将其分为对照组（6名健康志愿者，血清内毒素为0 U/mL）、实验组A （6例烧伤患者，血清内毒素为0～0?2 U/mL）、实验组B （6例烧伤患者，血清内毒素为0?2～0?4 U/mL）、实验组C （6例烧伤患者，血清内毒素＞0?4 U/mL）。用4组血清分别刺激正常人PBMCs不同时间后，用放射免疫分析法测定每组细胞培养上清液中的肿瘤坏死因子（ tumor necrosis factor， TNF）-α、白细胞介素（ interleukin， IL）-1β、 IL-6及IL-12的浓度，并进行数据分析。结果 TNF-α和IL-6在不同时间，被不同浓度血清内毒素刺激后均显著增加；随着刺激时间的延长， IL-1β和IL-12才显著增加。结论 TNF-α和IL-6为内毒素介导的烧伤炎症中重要的炎性介质，其浓度可随着时间的延长或内毒素浓度的升高而增加，但IL-1β和IL-12的浓度不随内毒素剂量的变化而变化，而是随着刺激时间的延长逐渐增加。
目的：探討燒傷患者血清內毒素對正常機體外週血單箇覈細胞（ peripheral blood mononuclear cells， PBMCs）中4種炎性細胞因子的影響。方法以正常人外週血單箇覈細胞為受試對象，以燒傷患者血清為刺激因素。根據18例燒傷患者與6名健康誌願者血清內毒素水平將其分為對照組（6名健康誌願者，血清內毒素為0 U/mL）、實驗組A （6例燒傷患者，血清內毒素為0～0?2 U/mL）、實驗組B （6例燒傷患者，血清內毒素為0?2～0?4 U/mL）、實驗組C （6例燒傷患者，血清內毒素＞0?4 U/mL）。用4組血清分彆刺激正常人PBMCs不同時間後，用放射免疫分析法測定每組細胞培養上清液中的腫瘤壞死因子（ tumor necrosis factor， TNF）-α、白細胞介素（ interleukin， IL）-1β、 IL-6及IL-12的濃度，併進行數據分析。結果 TNF-α和IL-6在不同時間，被不同濃度血清內毒素刺激後均顯著增加；隨著刺激時間的延長， IL-1β和IL-12纔顯著增加。結論 TNF-α和IL-6為內毒素介導的燒傷炎癥中重要的炎性介質，其濃度可隨著時間的延長或內毒素濃度的升高而增加，但IL-1β和IL-12的濃度不隨內毒素劑量的變化而變化，而是隨著刺激時間的延長逐漸增加。
목적：탐토소상환자혈청내독소대정상궤체외주혈단개핵세포（ peripheral blood mononuclear cells， PBMCs）중4충염성세포인자적영향。방법이정상인외주혈단개핵세포위수시대상，이소상환자혈청위자격인소。근거18례소상환자여6명건강지원자혈청내독소수평장기분위대조조（6명건강지원자，혈청내독소위0 U/mL）、실험조A （6례소상환자，혈청내독소위0～0?2 U/mL）、실험조B （6례소상환자，혈청내독소위0?2～0?4 U/mL）、실험조C （6례소상환자，혈청내독소＞0?4 U/mL）。용4조혈청분별자격정상인PBMCs불동시간후，용방사면역분석법측정매조세포배양상청액중적종류배사인자（ tumor necrosis factor， TNF）-α、백세포개소（ interleukin， IL）-1β、 IL-6급IL-12적농도，병진행수거분석。결과 TNF-α화IL-6재불동시간，피불동농도혈청내독소자격후균현저증가；수착자격시간적연장， IL-1β화IL-12재현저증가。결론 TNF-α화IL-6위내독소개도적소상염증중중요적염성개질，기농도가수착시간적연장혹내독소농도적승고이증가，단IL-1β화IL-12적농도불수내독소제량적변화이변화，이시수착자격시간적연장축점증가。
Objective To investigate the influence of endotoxins in serum of burn patients on 4 inflammatory cyto-kines in peripheral blood mononuclear cells of a normal person. Method The peripheral blood mononuclear cells of a nor-mal person were used as subjects for test and the serum of burn patients was used as a stimulus. According to the level of endotoxins contained in their serum, 18 burn patients and 6 healthy volunteers were divided into control group ( 6 healthy volunteers with a serum endotoxin level of 0 U/mL), test group A (6 burn patients with a serum endotoxin level of 0~0?2 U/mL), test group B (6 burn patients with a serum endotoxin level of 0?2~0?4 U/mL), and test group C (6 burn patients with a serum endotoxin level of over 0?4 U/mL) . The serum of all four groups were used respectively to stimulate PBMCs in cell culture for different length of time, after which the concentrations of TNF-α, IL-1β, IL-6 and IL-12 in supernatant of the cell culture of each group were determined by radioimmunoassay and data analysis was conducted. Result TNF-a and IL-6 increased remarkably after being stimulated by endotoxins of various concentrations for various length of time. IL-1βand IL-12 increased greatly only after prolonging the stimulating time. Conclusion TNF-a and IL-6 are two major inflam-matory media in endotoxin induced burn inflammation, their concentrations can increase with the prolonging of time or the increase of endotoxin’ s concentration. However, the concentrations of IL-1βand IL-12 do not change along with the change of endotoxin concentration, they increase gradually along with the prolonging of stimulating time.
