草原与草坪
草原與草坪
초원여초평
GRASSLAND AND TURF
2014年
2期
77-80,85
,共5页
向华%南丽丽%李春晓%王克婧%杜玺
嚮華%南麗麗%李春曉%王剋婧%杜璽
향화%남려려%리춘효%왕극청%두새
Pb2 + 胁迫%杂花苜蓿%种子萌发%生理特性
Pb2 + 脅迫%雜花苜蓿%種子萌髮%生理特性
Pb2 + 협박%잡화목숙%충자맹발%생리특성
lead stress%Medicago varia%seed germination%physiological characteristics
以杂花苜蓿阿尔冈金(Medicago varia cv.ALgonquin)为试验材料,采用沙培方法,研究了外源一氧化氮(NO)供体硝普钠(SNP)对 Pb2+处理下杂花苜蓿种子萌发及幼苗生理特性的影响。结果表明,与对照比,用 SNP(100μmol/L)、低 Pb2+浓度(250 mg/L)和 SNP 与低 Pb2+浓度组合(100μmol/L SNP +250 mg/L)处理均增加了杂花苜蓿种子的发芽势、发芽率、胚芽胚根长,提高了幼苗脯氨酸、可溶性糖及可溶性蛋白质的含量,增强了根系活力,降低了 MDA 含量。高 Pb2+浓度(500 mg/L)处理不利于杂花苜蓿种子萌发和幼苗生长,且100μmol/L SNP 不能缓解高铅胁迫对种子萌发及幼苗生长的抑制作用。
以雜花苜蓿阿爾岡金(Medicago varia cv.ALgonquin)為試驗材料,採用沙培方法,研究瞭外源一氧化氮(NO)供體硝普鈉(SNP)對 Pb2+處理下雜花苜蓿種子萌髮及幼苗生理特性的影響。結果錶明,與對照比,用 SNP(100μmol/L)、低 Pb2+濃度(250 mg/L)和 SNP 與低 Pb2+濃度組閤(100μmol/L SNP +250 mg/L)處理均增加瞭雜花苜蓿種子的髮芽勢、髮芽率、胚芽胚根長,提高瞭幼苗脯氨痠、可溶性糖及可溶性蛋白質的含量,增彊瞭根繫活力,降低瞭 MDA 含量。高 Pb2+濃度(500 mg/L)處理不利于雜花苜蓿種子萌髮和幼苗生長,且100μmol/L SNP 不能緩解高鉛脅迫對種子萌髮及幼苗生長的抑製作用。
이잡화목숙아이강금(Medicago varia cv.ALgonquin)위시험재료,채용사배방법,연구료외원일양화담(NO)공체초보납(SNP)대 Pb2+처리하잡화목숙충자맹발급유묘생리특성적영향。결과표명,여대조비,용 SNP(100μmol/L)、저 Pb2+농도(250 mg/L)화 SNP 여저 Pb2+농도조합(100μmol/L SNP +250 mg/L)처리균증가료잡화목숙충자적발아세、발아솔、배아배근장,제고료유묘포안산、가용성당급가용성단백질적함량,증강료근계활력,강저료 MDA 함량。고 Pb2+농도(500 mg/L)처리불리우잡화목숙충자맹발화유묘생장,차100μmol/L SNP 불능완해고연협박대충자맹발급유묘생장적억제작용。
Medicago varia cv.Algonquin was used to study the effects of nitric oxide donor SNP on seed germination and seedlings physiological characteristics under lead stress by sand culture.The results showed that the treatments with SNP of 100 μmol/L concentration,Pb of 250 mg/L concentration,and Pb of 250 mg/L and SNP of 100 μmol/L concentrations resulted in the improvement of germination potential,germination rate, root length,shoot length and root activity,and increasing of proline,soluble sugar and soluble protein contents, but the MDA content was decreased.High concentration Pb solution (500 mg/L)inhibited seedling growth and SNP (100 μmol/L)could not reduce the damage in this case.