CAJ | 학술논문

为明确燕麦散黑穗病菌(Ustilago avenae )冬孢子在自然生境中的萌发条件,研究了疏亲水界面、不同温度、湿度、光照时间、pH、碳源和氮源对冬孢子萌发的影响。结果表明,亲水界面促进冬孢子萌发,在水琼脂培养基上萌发率最高；冬孢子萌发的最适温度为20~25℃,温度过高或过低均抑制其萌发。冬孢子对湿度要求较高,RH≥90%时才可萌发,且萌发率较低。光照对冬孢子萌发的影响不明显,酸性条件有利于冬孢子萌发,最适 pH 为4~7,葡萄糖和蛋白胨可促进冬孢子萌发。
위명학연맥산흑수병균(Ustilago avenae )동포자재자연생경중적맹발조건,연구료소친수계면、불동온도、습도、광조시간、pH、탄원화담원대동포자맹발적영향。결과표명,친수계면촉진동포자맹발,재수경지배양기상맹발솔최고；동포자맹발적최괄온도위20~25℃,온도과고혹과저균억제기맹발。동포자대습도요구교고,RH≥90%시재가맹발,차맹발솔교저。광조대동포자맹발적영향불명현,산성조건유리우동포자맹발,최괄 pH 위4~7,포도당화단백동가촉진동포자맹발。
In the paper,we studied the effects of lyophobic and hydrophilic interface,different temperature, humidity,light illumination time,pH,carbon sources and nitrogen sources on Ustilago avenae teliospore germi-nation.The results showed that the hydrophilic interface could promote Ustilago avenae teliospore germination, The germination rate was highest on water agar medium;The optimum temperature of teliospore germination was from 20 to 25 ℃,Teliospore germination was inhibited by too high or too low on the temperature;The de-mand of humidity for teliospore was high,they could only germinate when the relative humidity was higher than 90%,and germination rate was low;The illumination time had not obvious effect on teliospore germination;A-cidic conditions could promote teliospore germination,the optimum pH was 4 to 7;Glucose and peptone could promote the teliospore germination.