CAJ | 학술논문

在完全营养液条件下,采用砂培法,研究了3种氮素形态配比 NO3--N,NH 4+-N 和 NO3--N∶NH 4+-N 为1∶1和0、105、210 mg/L 3个氮素水平对紫花苜蓿盛花期生长及结瘤固氮的影响。结果表明：不同形态氮素处理下紫花苜蓿的株高、生物量、根瘤数、根瘤重、固氮酶活性和全氮含量均显著高于CK。同一氮素水平下,株高表现为 NO3--N 和 NH 4+-N 混合培养下效果最好,NO3--N 培养下次之, NH 4+-N 培养下最低；生物量、根瘤数、根瘤重、固氮酶活性和全氮含量 NO3--N 和 NH 4+-N 混合培养下效果最好,NH 4+-N 培养下次之,NO3--N 培养下最低；茎叶比则是 NO3--N 培养下最大,NH 4+-N培养下次之,NO3--N 和 NH 4+-N 混合培养下的最低,各氮素形态处理间差异显著(P <0.05)。随着氮素水平的增加,各形态配比下紫花苜蓿的株高、生物量、根瘤数、根瘤重、固氮酶活性和全氮含量呈现增加的变化趋势,茎叶比呈现减小的变化趋势。NO3--N+NH 4+-N 的浓度为210 mg/L 时,紫花苜蓿的株高、生物量、根瘤数、固氮酶活性和全氮含量取得最大值,茎叶比取得最小值,根瘤重则在 NO3--N+NH 4+-N 的浓度为105 mg/L 时取得最大值,紫花苜蓿在混合态氮培养下生长最好。
재완전영양액조건하,채용사배법,연구료3충담소형태배비 NO3--N,NH 4+-N 화 NO3--N∶NH 4+-N 위1∶1화0、105、210 mg/L 3개담소수평대자화목숙성화기생장급결류고담적영향。결과표명：불동형태담소처리하자화목숙적주고、생물량、근류수、근류중、고담매활성화전담함량균현저고우CK。동일담소수평하,주고표현위 NO3--N 화 NH 4+-N 혼합배양하효과최호,NO3--N 배양하차지, NH 4+-N 배양하최저；생물량、근류수、근류중、고담매활성화전담함량 NO3--N 화 NH 4+-N 혼합배양하효과최호,NH 4+-N 배양하차지,NO3--N 배양하최저；경협비칙시 NO3--N 배양하최대,NH 4+-N배양하차지,NO3--N 화 NH 4+-N 혼합배양하적최저,각담소형태처리간차이현저(P <0.05)。수착담소수평적증가,각형태배비하자화목숙적주고、생물량、근류수、근류중、고담매활성화전담함량정현증가적변화추세,경협비정현감소적변화추세。NO3--N+NH 4+-N 적농도위210 mg/L 시,자화목숙적주고、생물량、근류수、고담매활성화전담함량취득최대치,경협비취득최소치,근류중칙재 NO3--N+NH 4+-N 적농도위105 mg/L 시취득최대치,자화목숙재혼합태담배양하생장최호。
Under the condition of complete nutrient solution,the effect of three kinds of nitrogen forms (ni-trate nitrogen,ammonium nitrogen and nitrate nitrogen,ammonium nitrogen was 1∶1 and three nitrogen levels (0,105,210 mg/L)on growth nodulation and nitrogen- fixation of alfalfa were studied at flowering stage by sand culture method.The results showed that different nitrogen treatments on plant height,biomass,nodule number,nodule weight,nitrogenase activity and total nitrogen content of alfalfa were significantly higher than those of CK.Under the same nitrogen level,the plant height was the highest and higher under the effect of NO3 --N and NH 4 +-N mixed culture and under NO3 --N next,and NH 4 +-N culture.The performance of bio-mass,nodule number,nodule weight,nitrogenase activity and total nitrogen content were the best under NO3 --N and NH 4 +-N mixed culture,it was the better and lowest under NH 4 +-N and NO3 --N culture respectively. The performance of stem leaf ratio was the best under NO3 --N training,it was better under NH 4 +-N training than that of NO3 --N and NH 4 +-N mixed culture.There was significant difference in nitrogen forms between treatments (P <0.05).Along with the increase of nitrogen level,the plant height,biomass,nodule number,nod-ule weight,nitrogenase activity and total nitrogen content of alfalfa were increased,the leaf stem ratio was de-creased.When the concentration of NO3 --N+NH 4 +-N was 210 mg/L,the plant height,biomass,nodule num-ber,nitrogenase activity and total nitrogen content of alfalfa reached the maximum value,the leaf stem ratio had a minimum value.The nodule weight reached the maximum value when the concentration of NO3 --N+NH 4 +-N was 105 mg/L.In conclusion,alfalfa grows best in mixed nitrogen culture.