CAJ | 학술논문

通过将兔多杀性巴氏杆菌C51-17株菌液分装后置-80℃冻存的方法对菌液浓度进行预数，并与传统方法采用的将菌液置4℃保存过夜计算菌存率或通过测定菌液吸光值推算攻毒菌液浓度相比较。结果表明，用-80℃冻存保存方法估算的菌液浓度与实际浓度更接近，采用这种方法进行疫苗的效力检验，能确保对照成立，在兔多杀性巴氏杆菌病灭活疫苗效力检验中可以采用-80℃冻存的方法进行攻毒菌液的预数。
통과장토다살성파씨간균C51-17주균액분장후치-80℃동존적방법대균액농도진행예수，병여전통방법채용적장균액치4℃보존과야계산균존솔혹통과측정균액흡광치추산공독균액농도상비교。결과표명，용-80℃동존보존방법고산적균액농도여실제농도경접근，채용저충방법진행역묘적효력검험，능학보대조성립，재토다살성파씨간균병멸활역묘효력검험중가이채용-80℃동존적방법진행공독균액적예수。
Pasteurella multocida strain C51-17 was required as the challenging strain in the assessment for the potency of rabbit Pasteurella multocida inactivated vaccine. In order to ensure the exactly challenging dosage, viable count for the bacteria liquid culture should be done before challenging to rabbits, which procedure was called pre-estimating. Traditional methods of pre-estimating viable count for the bacteria liquid culture were calculating bacteria survival rate stocked in 4℃ or measuring the culture absorption value. In this study, bacteria liquid culture was divided into portions and stocked in -80 ℃ as the challenging bacteria. These three kinds of dealing samples were used for pre-estimating viable count to compare the bacteria stability and vitality. The results indicated that bacteria liquid culture frozen in -80 ℃kept the best vitality and had good reproducibility, and it accorded with the challenging amount in the further viable count test. The bacteria culture dealt by this way could make the control established in each time in Pasteurella multocida inactivated vaccine potency test. Therefore, the method can be used to pre-estimating viable count of bacteria liquid culture for challenging in rabbit Pasteurella multocida inactivated vaccine potency test.