中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2014年
3期
47-49
,共3页
芩黄颗粒%甘草酸%高效液相色谱法
芩黃顆粒%甘草痠%高效液相色譜法
금황과립%감초산%고효액상색보법
Qinhuang Granula%glycyrrhizic acid%HPLC
为了建立芩黄颗粒中甘草酸的含量测定方法并测定其含量，用高效液相色谱法（ HP LC ）测定芩黄颗粒中甘草酸的含量。采用C18色谱柱，以甲醇-0．2 mol／L醋酸铵（65∶34，醋酸调节pH值至4．5）为流动相，流速为1．0 mL／min，检测波长为250 nm。结果显示甘草酸在0．499～7．976μg之间呈现良好的线性关系，r＝0．9999，平均加样回收率为99．3％，RSD＝0．59％（ n＝6）。本方法重复性好，能准确测定芩黄颗粒中甘草酸的含量。
為瞭建立芩黃顆粒中甘草痠的含量測定方法併測定其含量，用高效液相色譜法（ HP LC ）測定芩黃顆粒中甘草痠的含量。採用C18色譜柱，以甲醇-0．2 mol／L醋痠銨（65∶34，醋痠調節pH值至4．5）為流動相，流速為1．0 mL／min，檢測波長為250 nm。結果顯示甘草痠在0．499～7．976μg之間呈現良好的線性關繫，r＝0．9999，平均加樣迴收率為99．3％，RSD＝0．59％（ n＝6）。本方法重複性好，能準確測定芩黃顆粒中甘草痠的含量。
위료건립금황과립중감초산적함량측정방법병측정기함량，용고효액상색보법（ HP LC ）측정금황과립중감초산적함량。채용C18색보주，이갑순-0．2 mol／L작산안（65∶34，작산조절pH치지4．5）위류동상，류속위1．0 mL／min，검측파장위250 nm。결과현시감초산재0．499～7．976μg지간정현량호적선성관계，r＝0．9999，평균가양회수솔위99．3％，RSD＝0．59％（ n＝6）。본방법중복성호，능준학측정금황과립중감초산적함량。
To establish the method for determining of glycyrrhizic acid in Qinhuang Granula by HPLC, C18 column was used with the mobile phase consisted of methanol-0.2mol/L ammonium acetate (65 ∶ 34, adjusted pH to 4.5 with CH3 COOH) , the flow rate was 1.0 mL/min, the detection wavelength was 250 nm. The calibration curve was linear at a range of 0. 499~7. 977 μg for glycyrrhizic acid ( r=0. 9999 ) , the average recovery was 99.3%and RSD was 0.59%(n=6). The method was accurate with a good reproducibility and can be used as a quantitative analysis of glycyrrhizic acid in Qinhuang Granula.
