CAJ | 학술논문

建立了超高效液相色谱-串联质谱法并对克伦特罗（ Clenbuterol ）在绒山羊体内残留消除规律进行了研究。选取60只健康绒山羊（30±4．8）kg，平均分成2组，分别在饲料中添加150μg／kg b．w．（A组）和300μg／kg b．w．（B组）克伦特罗，连续饲喂25 d后停药。建立UPLC-MS／MS检测方法监测喂药期尿液、休药期尿液和组织中克伦特罗含量。克伦特罗在饲喂期间，A组尿液中平均含量为289～5313μg／L，B组尿液中平均含量为1323～11378μg／L。停药后尿液中克伦特罗含量迅速下降，停药40 d后，A、B两组均下降至10μg／L以下；停药后，取绒山羊肝脏、肾脏、肺脏、肌肉测定其残留量。 A、B两组停药后组织中克伦特罗含量持续下降，停药40 d后，肝脏含量33μg／kg，肾脏约5μg／kg，其余组织均未检出。
건립료초고효액상색보-천련질보법병대극륜특라（ Clenbuterol ）재융산양체내잔류소제규률진행료연구。선취60지건강융산양（30±4．8）kg，평균분성2조，분별재사료중첨가150μg／kg b．w．（A조）화300μg／kg b．w．（B조）극륜특라，련속사위25 d후정약。건립UPLC-MS／MS검측방법감측위약기뇨액、휴약기뇨액화조직중극륜특라함량。극륜특라재사위기간，A조뇨액중평균함량위289～5313μg／L，B조뇨액중평균함량위1323～11378μg／L。정약후뇨액중극륜특라함량신속하강，정약40 d후，A、B량조균하강지10μg／L이하；정약후，취융산양간장、신장、폐장、기육측정기잔류량。 A、B량조정약후조직중극륜특라함량지속하강，정약40 d후，간장함량33μg／kg，신장약5μg／kg，기여조직균미검출。
The depletion rules of clenbuterol in urine and tissues after treatment were studied by the developed UPLC/MS/MS method. 60 goats(30±4.8)kg were divided into 2 groups named group A and B .Goats were fed 150 μg/kg b.w.(group A)and 300 μg/kg b.w.(group B) for twenty five consecutive days.Samples of urine and tissues were collected to determine clenbuterol concentration by UPLC-MS/MS. The results showed that the mean concentrations of clenbuterol were 289~5313 μg/L and 1323~11378 μg/L in group A and B in urine during feeding period. The concentrations of clenbuterol in urine rapidly decreasd under 10 μg/L during the withdrawal period. After a 40-day withdrawal period, the mean concentrations of clenbuterol were 33 μg/kg in liver and 5μg/kg kidney, but the concentration of muscle and lung were not detected.