CAJ | 학술논문

为了优化苦参碱的提取工艺，以浸膏收率、苦参碱含量为评价指标，选择用水量、提取时间及提取次数为考察因素，利用L9（34）正交试验法优选出水煎煮法提取苦参碱的最佳工艺条件为用8倍量水提取3次，每次提取2h。采用乙醇沉淀及三氯甲烷萃取相结合的方法对苦参粗提物进行纯化，浓缩药液浓度为1．0 g生药／mL时进行醇沉，醇沉后调节药液的pH值至9～11用三氯甲烷萃取3次。优选出的提取工艺稳定、可行，可用于苦参碱的工业化提取。
위료우화고삼감적제취공예，이침고수솔、고삼감함량위평개지표，선택용수량、제취시간급제취차수위고찰인소，이용L9（34）정교시험법우선출수전자법제취고삼감적최가공예조건위용8배량수제취3차，매차제취2h。채용을순침정급삼록갑완췌취상결합적방법대고삼조제물진행순화，농축약액농도위1．0 g생약／mL시진행순침，순침후조절약액적pH치지9～11용삼록갑완췌취3차。우선출적제취공예은정、가행，가용우고삼감적공업화제취。
To optimize extraction process of alkaloids from S. flavescens, taking alkaloids from S.flavescens and dry extract as indexes, orthogonal design was used to investigate effect of solvent consumption, extraction time, extraction times on extraction process. Optimal extraction process was: extracted 3 times with 8 times amount of water, 2 h each time. Crude extractings from sophora were purified by ethanol precipitation and chloroform extraction, concentrating extracting solution to 1.0 g medicinal materials per milliliter before ethanol precipitation and adjusting the pH of the solution to 9~11, chloroform extraction 3 times. This optimized technology was stable and feasible, it could be used to industrial extraction of alkaloids from S. flavescens.