CAJ | 학술논문

为分析仔猪体内猪瘟母源抗体的衰减规律及母源抗体保护水平，测定了断奶时母猪和仔猪抗体水平，并监测仔猪母源抗体水平至81日龄；分别对12头81日龄仔猪（来自5头母猪）和25头35日龄仔猪（来自5头母猪）进行了攻毒试验，并用RT-nPCR进行了跟踪检测，对7头攻毒耐过猪用兔体中和法测定了中和效价。结果表明，母猪抗体水平不均衡会导致仔猪群体母源抗体水平不均衡；仔猪群体抗体到60日龄时仍然有67％（12／18）的个体用ELISA检测阻断率在50％以上；81日龄攻毒组有3头（3／12）耐过，35日龄攻毒组能全部耐过（25／25），但有2头（2／25）在攻毒27 d后仍然呈PCR阳性，此时中和抗体水平从低于1∶4到1∶16不等，推测中和抗体水平1∶8～1∶16是母源抗体保护的临界线。
위분석자저체내저온모원항체적쇠감규률급모원항체보호수평，측정료단내시모저화자저항체수평，병감측자저모원항체수평지81일령；분별대12두81일령자저（래자5두모저）화25두35일령자저（래자5두모저）진행료공독시험，병용RT-nPCR진행료근종검측，대7두공독내과저용토체중화법측정료중화효개。결과표명，모저항체수평불균형회도치자저군체모원항체수평불균형；자저군체항체도60일령시잉연유67％（12／18）적개체용ELISA검측조단솔재50％이상；81일령공독조유3두（3／12）내과，35일령공독조능전부내과（25／25），단유2두（2／25）재공독27 d후잉연정PCR양성，차시중화항체수평종저우1∶4도1∶16불등，추측중화항체수평1∶8～1∶16시모원항체보호적림계선。
In order to analyze classical swine fever maternal antibodies’ decreasing pattern and protective level, antibodies of sows and piglets were detected at weaning day, maternal antibodies of piglets were monitored until 81 days after birth. Moreover, 12 81-day piglets from 5 sows and 25 35-day piglets from another 5 sows were challenged by SM strain and furthermore were monintored with RT nPCR. Antibody of 7 piglets surviving from challenge was detected with rabbit neutralization test. The results showed that antibodies of sows were correlative to that of piglets, antibody uniformity of sows led to the uniformity of piglets, blocking rates by ELISA were more than 50% in 67%(12/18) 60-day piglets. After challenging, 3 81-day piglets(3/12) and all 35-day piglets (25/25) could survive, and 2 35-day piglets ( 2/25 ) were nucleotide positive by RT-nPCR detection. The neutralization antibody titers were from less than 1 ∶ 4 to 1 ∶ 16. The data suggested that neutralization antibody titers 1 ∶ 8 to 1 ∶ 16 might be the threshold level of protection.