CAJ | 학술논문

为获得能产生高效蛋白酶的新菌株，应用于洗涤、食品、饲料加工及医药等领域，由短蛸的胃肠道中分离筛选出10种胃肠道菌株，其中4种菌S-1、S-2、S-3和S-8的蛋白酶活性较高，对其产生的蛋白酶进行生化特性的研究。结果显示，这4种菌株发酵产粗酶液的酶活性分别为1289、937、1222、885 U／m L ；S-1和S-3蛋白酶的最佳反应温度为30℃，S-2和S-8为50℃；蛋白酶最佳反应p H值分别为8．5、7．0、8．0和7．0。通过SDS-PAGE测定，并以酪蛋白为底物进行活性染色，发现粗酶溶液活性高的S-1菌株产生的主要蛋白酶分子量分别为149．6、127．4、40．3 ku；S-3菌株产生的主要蛋白酶分子量分别为123．3、112．2、71．6 ku和37．8 ku。研究表明，从短蛸胃肠道中可分离出产蛋白酶菌株的发酵条件容易实现，产生的蛋白酶活力较高，性质稳定，可应用于工业生产。
위획득능산생고효단백매적신균주，응용우세조、식품、사료가공급의약등영역，유단소적위장도중분리사선출10충위장도균주，기중4충균S-1、S-2、S-3화S-8적단백매활성교고，대기산생적단백매진행생화특성적연구。결과현시，저4충균주발효산조매액적매활성분별위1289、937、1222、885 U／m L ；S-1화S-3단백매적최가반응온도위30℃，S-2화S-8위50℃；단백매최가반응p H치분별위8．5、7．0、8．0화7．0。통과SDS-PAGE측정，병이락단백위저물진행활성염색，발현조매용액활성고적S-1균주산생적주요단백매분자량분별위149．6、127．4、40．3 ku；S-3균주산생적주요단백매분자량분별위123．3、112．2、71．6 ku화37．8 ku。연구표명，종단소위장도중가분리출산단백매균주적발효조건용역실현，산생적단백매활력교고，성질은정，가응용우공업생산。
Ten bacterial strains were isolated from octopus Octopus ocellatus by casein medium ,in which 4 strains S-1 ,S-2 ,S-3 and S-8 showed high protease activity .The major physical and chemical characteristics of the proteinases were studied in this article .The protease activity of the fermentation liquid was found to be 1289 U/mL in S-1 ,937 U/mL in S-2 ,1222 U/mL in S-3 ,and 885 U/mL in S-8 . The maximal proteases activity was observed at temperature of 30 ℃ for the strains S-1 ,and S-3 ,and at 50 ℃ for the strains S-2 ,and S-8 ,with the optimum pH of 8 .5 in stain S-1 ,7 .0 in strain S-2 ,8 .0 in S-3 ,and 7 .0 in S-8 .The SDS-PAGE ,substrate casein and active staining confirmed that the main proteases produced by strain S-1 had molecular weights of 149 .6 ,127 .4 and 40 .3 ku ,and the protease molecular mass in strain S-3 showed molecular weights of about 123 .3 ,112 .2 ,71 .6 and 37 .8 ku .The findings suggest that the protease from protease-producing bacterial strains in the octopus was feasible to be isolated due to suitable fermentation condition ,high activity and stability and can be applied to industrial production .