CAJ | 학술논문

自海南水产养殖水体中分离筛选获得1株具有高效改良水质功能的益生菌株B t-01，经菌落形态和生理生化分析及16S rDNA鉴定，该菌株为苏云金芽孢杆菌。采用腹腔注射法检测其安全性，测定该菌株对静养水体化学需氧量、亚硝酸盐和氨氮的相对移除效率。试验结果表明，腹腔注射4．1×108 cfu／mL的Bt-01菌悬液200μL ，养殖的尼罗罗非鱼30 d成活率为100％；Bt-01在12 h内对亚硝酸盐相对移除率最高可达98．03％，31 h内对氨氮的相对移除率为59．10％，23．5 h内对化学需氧量的相对移除率最高可达51．06％。该菌株具有显著改良水产养殖水质作用和良好应用前景。
자해남수산양식수체중분리사선획득1주구유고효개량수질공능적익생균주B t-01，경균락형태화생리생화분석급16S rDNA감정，해균주위소운금아포간균。채용복강주사법검측기안전성，측정해균주대정양수체화학수양량、아초산염화안담적상대이제효솔。시험결과표명，복강주사4．1×108 cfu／mL적Bt-01균현액200μL ，양식적니라라비어30 d성활솔위100％；Bt-01재12 h내대아초산염상대이제솔최고가체98．03％，31 h내대안담적상대이제솔위59．10％，23．5 h내대화학수양량적상대이제솔최고가체51．06％。해균주구유현저개량수산양식수질작용화량호응용전경。
A bacterium with highly effective water purification named as Bt-01 has been screened out from aquaculture area in Hainan province and was identified as Bacillus thuringiensis by colonial morphology , macro-morphology , biochemical characteristics and 16S rDNA .The safety of Bt-01 was studied by intraperitoneal injection ,and the removal efficiency of COD ,nitrite nitrogen and ammonia nitrogen by Bt-01 were investigated in tilapia culturing water .The results showed that the survival rate of 100% was observed in the tilapia injected intraperitonally with 200μL bacterial suspension and then cultured for 30 d . The removal efficiency of nitrite nitrogen was found to be the maximum value of 98 .03% in the water for 12 h ,and 59 .10% for 31 h .The maximal COD removal efficiency of 51 .06% was found in the culturing tilapia water for 23 .5 h .The findings reveal that Bt-01 shows highly effective removal of COD ,nitrite nitrogen and ammonia nitrogen in aquaculture water and would be popular in aquaculture industry .