CAJ | 학술논문

血细胞处理仪的改进研究
혈세포처리의적개진연구
Protocols of improving red blood cells processing devices

万方数据

军事医学軍事醫學 군사의학
BULLETIN OF THE ACADEMY OF MILITARY MEDICAL SCIENCES
2014年 4期 298-300 ,共3页

刘敏霞%张长虹%杨周伟%王艳%谷涛%王捷熙%杜为%杨超%周俊%吴涛%韩颖

류민하%장장홍%양주위%왕염%곡도%왕첩희%두위%양초%주준%오도%한영

冰冻红细胞%血细胞处理仪%红细胞质量%低温保存冰凍紅細胞%血細胞處理儀%紅細胞質量%低溫保存
빙동홍세포%혈세포처리의%홍세포질량%저온보존
frozen red blood cells%red blood cells processing device%red blood cell quality%cryopreservation

目的：对血细胞处理仪（ BBS926型全自动医用低速离心机）处理冰冻红细胞技术进行改进研究，确定冰冻红细胞洗涤程序和效果。方法取源于400 ml （2 U）全血的健康献血者悬浮红细胞，-80℃冰冻保存，采用血细胞处理仪对解冻红细胞进行处理，在初始程序（程序1）洗涤冰冻红细胞的基础上，通过对洗液量和洗涤步骤等进行改进，形成新的优化程序（程序2）洗涤冰冻红细胞；对两组程序洗涤后的冰冻红细胞质量进行评价。结果两组洗涤程序（程序1和程序2）处理后的冰冻红细胞质量各项指标检测：血红蛋白（Hb）含量（g）分别为37．55±3．58和42．18±3．35（P＜0．05），游离血红蛋白（FHb）含量（g/L）分别为0．51±0．08和0．53±0．07（P＞0．05），白细胞残留量（×107个）分别为1．90±0．99和1．92±1．04（P＞0．05），晶体渗透压（mOsm）分别为334±8．03和327±9．06（P＞0．05），无菌实验普通细菌和真菌检测均为阴性。红细胞体外溶血率（％）分别为12．44±8．24和12．02±5．78（P＞0．05），红细胞变形性（EI）分别为21．40±1．41和21．42±1．45（P＞0．05），红细胞回收率（％）分别为72．02±3．70和77．18±5．58（P＜0．05），红细胞凋亡（％）分别为1．12±0．54和1．10±0．61（P＞0．05），洗涤时间（min）分别为79．00±0．71和79．60±0．55（P＞0．05）。结论两组洗涤程序洗涤冰冻红细胞质量均达到国家冰冻解冻去甘油红细胞质量控制要求，改进后洗涤程序2处理的冰冻红细胞Hb含量和红细胞回收率有一定提高；程序2洗涤效果优于程序1。
목적：대혈세포처리의（ BBS926형전자동의용저속리심궤）처리빙동홍세포기술진행개진연구，학정빙동홍세포세조정서화효과。방법취원우400 ml （2 U）전혈적건강헌혈자현부홍세포，-80℃빙동보존，채용혈세포처리의대해동홍세포진행처리，재초시정서（정서1）세조빙동홍세포적기출상，통과대세액량화세조보취등진행개진，형성신적우화정서（정서2）세조빙동홍세포；대량조정서세조후적빙동홍세포질량진행평개。결과량조세조정서（정서1화정서2）처리후적빙동홍세포질량각항지표검측：혈홍단백（Hb）함량（g）분별위37．55±3．58화42．18±3．35（P＜0．05），유리혈홍단백（FHb）함량（g/L）분별위0．51±0．08화0．53±0．07（P＞0．05），백세포잔류량（×107개）분별위1．90±0．99화1．92±1．04（P＞0．05），정체삼투압（mOsm）분별위334±8．03화327±9．06（P＞0．05），무균실험보통세균화진균검측균위음성。홍세포체외용혈솔（％）분별위12．44±8．24화12．02±5．78（P＞0．05），홍세포변형성（EI）분별위21．40±1．41화21．42±1．45（P＞0．05），홍세포회수솔（％）분별위72．02±3．70화77．18±5．58（P＜0．05），홍세포조망（％）분별위1．12±0．54화1．10±0．61（P＞0．05），세조시간（min）분별위79．00±0．71화79．60±0．55（P＞0．05）。결론량조세조정서세조빙동홍세포질량균체도국가빙동해동거감유홍세포질량공제요구，개진후세조정서2처리적빙동홍세포Hb함량화홍세포회수솔유일정제고；정서2세조효과우우정서1。
Objective To improve the protocols of red blood cells ( RBCs) processing devices ( automatic medical RBC centrifuge, type:BBS926).Methods RBCs separated from 400 ml of whole blood collected from healthy donors were frozen at -80℃.After thawing , the cells were processed by the washing device .Based on the original protocol ( protocol 1), a modified protocol (protocol 2) was established and used to evaluate the quality of the frozen RBCs .In the test group (protocol 2), the amount of washing buffers and the washing steps were revised to form the optimized protocol .RBCs processed with the two protocols were evaluated by different assays .Results The indexes from the standards for frozen-thawed RBCs: the amount of hemoglobin ( Hb) of RBCs from protocol 1 and protocol 2 was 37.55 ±3.58 and 42.18 ±3.35 g(P<0.05),respectively;the amount of free hemoglobin(FHb) was 0.51 ±0.08 g/L and 0.53 ±0.07 g/L (P>0.05);the residual amount of white blood cells (WBCs) was (1.90 ±0.99) ×107 and (1.92 ±1.04) ×107(P>0.05);The osmolarities were 334 ±8.03 mOsm and 327 ±9.06 mOsm(P>0.05);both the bacteria and fungi tests were negative for the RBCs processed with the two protocols .Among other indexes ,the hemolysis rate for RBCs from protocol 1 and protocol 2 was (12.44 ±8.24)%and (12.02 ±5.78)%(P>0.05), the deformation index was 21.40 ±1.41 and 21.42 ±1.45 (P>0.05), the RBC recovery was(72.02 ±3.70)%and (77.18 ±5.58)%(P<0.05),the cell apopto-sis rate was(1.12 ±0.54)%and (1.10 ±0.61)%(P>0.05),and the processing time was (79.00 ±0.71)min and (79.60 ±0.55)min (P>0.05).Conclusion The RBCs processed by the two protocols meet the national standards for frozen-thaw RBCs.Hb amounts and cell recoveries of the RBCs are enhanced by treatment with protocol 2.Protocol 2 proves to be better than protocol 1.