CAJ | 학술논문

目的：系统观察不同军事应激模式和军事应激负荷下大鼠血清中重要应激相关激素的变化规律。方法以改良水环境多平台睡眠剥夺法（ MMPM）建立睡眠剥夺模型；以自制束缚仓建立大鼠束缚应激模型；以控制动作、使笼潮湿、使笼倾斜、通宵照明、禁食禁水、空瓶刺激、成组饲养等7种温和应激方式建立大鼠慢性不可预计温和应激（ CUMS）模型；分别检测上述3种应激模式下不同应激负荷大鼠血清中皮质醇、儿茶酚胺类和5-HT水平的变化规律。结果睡眠剥夺5d和7d大鼠血清GC含量显著高于对照组，而血清儿茶酚胺类含量则在睡眠剥夺1d显著升高；CUMS干预4周后大鼠血浆GC显著高于对照组，血清5-HT水平则显著低于对照组，血清儿茶酚胺类含量在2～8周均显著高于对照组；束缚应激大鼠1周后儿茶酚胺类显著升高，3～4周后则恢复到正常水平，而GC含量则在2～4周显著高于对照组。结论在不同的应激模式下大鼠血清GC水平随应激负荷增加而逐渐升高，可以作为评价不同应激模式下应激负荷的激素指标；儿茶酚胺类和5-HT则不具备作为类似指标的特征。
목적：계통관찰불동군사응격모식화군사응격부하하대서혈청중중요응격상관격소적변화규률。방법이개량수배경다평태수면박탈법（ MMPM）건립수면박탈모형；이자제속박창건립대서속박응격모형；이공제동작、사롱조습、사롱경사、통소조명、금식금수、공병자격、성조사양등7충온화응격방식건립대서만성불가예계온화응격（ CUMS）모형；분별검측상술3충응격모식하불동응격부하대서혈청중피질순、인다분알류화5-HT수평적변화규률。결과수면박탈5d화7d대서혈청GC함량현저고우대조조，이혈청인다분알류함량칙재수면박탈1d현저승고；CUMS간예4주후대서혈장GC현저고우대조조，혈청5-HT수평칙현저저우대조조，혈청인다분알류함량재2～8주균현저고우대조조；속박응격대서1주후인다분알류현저승고，3～4주후칙회복도정상수평，이GC함량칙재2～4주현저고우대조조。결론재불동적응격모식하대서혈청GC수평수응격부하증가이축점승고，가이작위평개불동응격모식하응격부하적격소지표；인다분알류화5-HT칙불구비작위유사지표적특정。
Objective To systematially observe the different patterns and leves of military stress on modified multitle-platform method the level of stress-related hormones in rats .Methods A sleep deprivation model was established by water environment modified multiple-platform method(MMPM).A restraint stress model was established by self-made bondage cage.Chronic unpredictable mild stress ( CUMS) model was established by action control , cage damp, cage tilt, night lightening, water and food fasting , empty bottles stimulation and group feeding methods .Cortisol, catecholamines and 5-HT levels were detected to observe the different military stress loads and modes on the level of stress hormones .Results The level of serum cortisol increased significantly ( P <0.05 ) after 5 days of sleep deprivation .The level of serum catecholamines increased significantly (P<0.05) after 1 day of sleep deprivation.The cortisol concentration increased and the level of 5-HT decreased in serum after 4 weeks CUMS.The level of catecholamines increased significantly (P<0.05) after 2-8 weeks of CUMS, increased significantly (P<0.05) after 1 week of restraint stress , and returned to normal after 3-4 weeks of restraint stress .The level of serum cortisol increased significantly after 3 -4 weeks of restraint stress . Conclusion Cortisol levels gradually increased with the level of stress in different military stress modes , which can serve as an index to evaluate the level of different stress modes .