CAJ | 학술논문

生物的分类是认知自然界的基础，动物 DNA 条形码是利用线粒体 COI 基因部分片段的序列差异鉴定物种的一种新技术，近年来在昆虫及其他动物类群中开展了大量的验证性和应用性研究。概述了动物线粒体基因适宜于鉴别物种的对应特征，回顾了 DNA 条形码背景，总结了 DNA 条形码技术的识别原理和鉴定程序，并对传统害虫形态鉴定方法的缺陷以及 DNA 条形码技术在害虫鉴定中的应用进展进行了综述，展望了 DNA 条形码研究中存在的争议和应用前景。
생물적분류시인지자연계적기출，동물 DNA 조형마시이용선립체 COI 기인부분편단적서렬차이감정물충적일충신기술，근년래재곤충급기타동물류군중개전료대량적험증성화응용성연구。개술료동물선립체기인괄의우감별물충적대응특정，회고료 DNA 조형마배경，총결료 DNA 조형마기술적식별원리화감정정서，병대전통해충형태감정방법적결함이급 DNA 조형마기술재해충감정중적응용진전진행료종술，전망료 DNA 조형마연구중존재적쟁의화응용전경。
The classification of living things is fundamental to the cognition of biological science. Animal DNA Barcoding is a new method for the identification of species in a wide range of animal taxa, which uses the partial region of the mitochondrial cytochrome coxidase-I (CO-I).In recent years,Growing researches are focused on its value and application.In this review,the background,identification principle and procedure of DNA barcoding were summarized.The weakness of the traditional approaches and the progress of application on pest identification by DNA barcoding were reviewed.At last,the existing disputes and future research direction of DNA barcoding were proposed.