CAJ | 학술논문

目的探讨慢性丙型肝炎(CHC)患者体内氧化损伤的情况.方法 52例CHC患者,按丙氨酸转氨酶(ALT)水平分为A组(ALT上升组)和B组(ALT正常组).正常对照组为20例健康志愿者.利用酶联免疫吸附法(ELISA)测定研究对象血清黄嘌呤氧化酶(XOD),丙二醛(MDA),氧化型谷胱甘肽(GSSG),谷胱甘肽(GSH),谷胱甘肽过氧化物酶(GSH-Px),谷胱甘肽巯基转移酶(GST),谷胱甘肽还原酶(GR)及维生素C(Vc)水平,并作出统计分析.结果 CHC患者血清XOD,MDA,GST和GR水平较正常对照组显著升高,而GSH,GSH-Px和Vc水平则明显降低.同时,A组患者血清XOD,MDA,GSSG,GST及GR水平较B组患者显著上调,而GSH,GSH-Px和Vc水平则显著下调.在CHC患者中,血清XOD,MDA,GSSG,GST水平与ALT水平呈正相关,血清GSH,GSH-Px,Vc与ALT水平呈负相关；血清XOD,MDA,GSSG,GR,GST水平与AST水平呈正相关,血清GSH-Px水平与AST水平呈负相关；血清GR水平与GGT水平呈正相关,血清GSH水平与GGT水平呈负相关；血清MDA,GR水平与AKP水平呈正相关.在CHC组中,仅血清XOD水平与血清HCV RNA水平间存在正相关关系.结论 CHC患者体内存在一定程度的氧化损伤,随血清ALT水平的升高,机体氧化损伤程度进一步加重.
목적 탐토만성병형간염(CHC)환자체내양화손상적정황.방법 52례CHC환자,안병안산전안매(ALT)수평분위A조(ALT상승조)화B조(ALT정상조).정상대조조위20례건강지원자.이용매련면역흡부법(ELISA)측정연구대상혈청황표령양화매(XOD),병이철(MDA),양화형곡광감태(GSSG),곡광감태(GSH),곡광감태과양화물매(GSH-Px),곡광감태구기전이매(GST),곡광감태환원매(GR)급유생소C(Vc)수평,병작출통계분석.결과 CHC환자혈청XOD,MDA,GST화GR수평교정상대조조현저승고,이GSH,GSH-Px화Vc수평칙명현강저.동시,A조환자혈청XOD,MDA,GSSG,GST급GR수평교B조환자현저상조,이GSH,GSH-Px화Vc수평칙현저하조.재CHC환자중,혈청XOD,MDA,GSSG,GST수평여ALT수평정정상관,혈청GSH,GSH-Px,Vc여ALT수평정부상관；혈청XOD,MDA,GSSG,GR,GST수평여AST수평정정상관,혈청GSH-Px수평여AST수평정부상관；혈청GR수평여GGT수평정정상관,혈청GSH수평여GGT수평정부상관；혈청MDA,GR수평여AKP수평정정상관.재CHC조중,부혈청XOD수평여혈청HCV RNA수평간존재정상관관계.결론 CHC환자체내존재일정정도적양화손상,수혈청ALT수평적승고,궤체양화손상정도진일보가중.
Objective This study is aimed to investigate oxidative stress status in chronic hepatitis C (CHC) patients.Methods 52 CHC patients were divided into two groups according to the serum level of alanine aminotransferase (ALT):group A (elevated ALT group) and group B (normal ALT group).20 healthy controls were included in this study.Serum levels of xanthine oxidase (XOD),malondialdehyde (MDA),oxidizided glutathione (GSSG),glutathione (GSH),glutathione peroxidase (GSH-Px),glutathione S-transferase (GST),glutathione reductase (GR) and vitamin C (Vc) were determined by.enzyme-linked immunosorbent assay (ELISA).Results Serum levels of XOD,MDA,GST and GR increased in CHC patients compared with healthy controls.While,serum levels of GSH,GSH-Px and Vc decreased compared with healthy controls.Furthermore,serum levels of XOD,MDA,GSSG,GST and GR in group A were up-regulated compared with group B.Serum levels of GSH,GSH-Px and Vc in group A were down-regulated compared with group B.In CHC patients,serum ALT level positively correlated with serum levels of XOD,MDA,GSSG and GST,while,negatively correlated with serum levels of GSH,GSH-Px and Vc.Serum aspartate aminotransferase (AST) level positively correlated with serum levels of XOD,MDA,GSSG,GR and GST,while,negatively correlated with serum GSH-Px level in CHC patients.Serum gamma-glutamyl transpeptidase (GGT) level positively correlated with serum GR level and negatively correlated with serum GSH level in CHC patients.Serum alkaline phosphatase (AKP) level positively correlated with serum levels of MDA and GR in CHC patients.In CHC patients,serum XOD level was positively related with serum HCV RNA level.Conclusion Oxidative stress was increased in CHC patients.In CHC patients with elevated serum ALT level,oxidative stress usually became serious.