CAJ | 학술논문

为获取脱病毒吊瓜组培苗，实现大规模优质吊瓜种苗生产，以越蒌四号带毒吊瓜组培苗为材料，研究恒温、变温热处理时间和茎尖大小对组培苗生长及茎尖苗脱毒效果的影响。结果表明，处理时间相同时变温热处理比恒温热处理组培苗的成活率高，越蒌四号吊瓜组培苗宜选择变温热处理5～15 d，试管苗的成活率在90％～100％；在变温热处理时间相同时，茎尖越小，脱毒率越高，但成活率也越低。综合考虑认为，在保证成活率和脱毒率条件下，越蒌四号吊瓜宜选用变温热处理10 d 后剥取0．2～0．4 mm 的茎尖进行培养，此方法成活率可达60％，脱毒率达100％。
위획취탈병독조과조배묘，실현대규모우질조과충묘생산，이월루사호대독조과조배묘위재료，연구항온、변온열처리시간화경첨대소대조배묘생장급경첨묘탈독효과적영향。결과표명，처리시간상동시변온열처리비항온열처리조배묘적성활솔고，월루사호조과조배묘의선택변온열처리5～15 d，시관묘적성활솔재90％～100％；재변온열처리시간상동시，경첨월소，탈독솔월고，단성활솔야월저。종합고필인위，재보증성활솔화탈독솔조건하，월루사호조과의선용변온열처리10 d 후박취0．2～0．4 mm 적경첨진행배양，차방법성활솔가체60％，탈독솔체100％。
The tissue culture plantlets with toxicity of Yuelou 4 (Trichosanthes kirilowii variety)were treated under constant and variable temperature for different days to study effects of constant and variable temperature treatment on growth of the tissue culture plantlets and the effect of variable temperature treatment and stem apex size on stem apex detoxification by RT-PCR.The results showed that the survival rate of tissue culture plantlets under variable temperature was higher than that under constant temperature for the same treatment days and the survival rate of tissue culture plantlets under variable temperature for 5-15 d was up to 90%~100%.The smaller the stem apex,the higher the detoxification rate under variable temperature for the same days but the lower the survival rate.The survival rate and detoxification rate of stem apex with 0.2~0.4 mm isolated from plantlets under variable temperature treatment for 10d could reach 60% and 100% respectively in view of rational survival rate and detoxification rate.