CAJ | 학술논문

为寻求抗ALV-J感染雏鸡用药物，通过CCK-8试剂盒检测细胞活力以确定利巴韦林、病毒灵和黄芪多糖对DF1细胞的最大安全浓度，用ELISA试剂盒检测ALV-J P27抗原表达量，以确定上述抗病毒药物体外抗ALV-J活性。结果显示，利巴韦林、病毒灵和黄芪多糖对DF1细胞的最大安全浓度分别为0．0391、0．0625和0．0625 mg／mL。在安全范围内，利巴韦林对P27的表达量具有明显的抑制作用，黄芪多糖在0．0039～0．0625 mg／mL安全浓度范围内明显抑制P27的表达，病毒灵在安全范围内对P 27的表达基本上没有抑制作用。试验表明，利巴韦林和黄芪多糖对ALV-J的增殖有明显的抑制作用，有望作为该病净化的辅助性防制药物。
위심구항ALV-J감염추계용약물，통과CCK-8시제합검측세포활력이학정리파위림、병독령화황기다당대DF1세포적최대안전농도，용ELISA시제합검측ALV-J P27항원표체량，이학정상술항병독약물체외항ALV-J활성。결과현시，리파위림、병독령화황기다당대DF1세포적최대안전농도분별위0．0391、0．0625화0．0625 mg／mL。재안전범위내，리파위림대P27적표체량구유명현적억제작용，황기다당재0．0039～0．0625 mg／mL안전농도범위내명현억제P27적표체，병독령재안전범위내대P 27적표체기본상몰유억제작용。시험표명，리파위림화황기다당대ALV-J적증식유명현적억제작용，유망작위해병정화적보조성방제약물。
To acquire the anti-ALV-J drug, P27 expression quantity influence of Ribavirin, moroxydine and astragalan on ALV-J ( NX0101 ) are researched by the CCK 8 kits cell detected for the maximum safe concentrations of the three antiviral drugs on DF1 cell and ALV-J P27 antigen ELISA test kit for P27 expression quantity.The results show that the maximum safe concentrations of Ribavirin, moroxydine and astragalan on DF1 cell are 0. 039, 0. 0625 and 0. 0625 mg/mL. The obvious inhibitory concentration of Ribavirin for the P27 expression quantity in the the scale of safe concentrations is 0.0391 mg/mL. The concentration range of astragalan inhibiting the expression of ALV-J P27 are 0.0039~0.0625 mg/mL. However, varied concentration of moroxydine in the research has no inhabitory effect on P27 expression quantity. The aforementioned results show that ribavirin and astragalan can inhabit the propogation of ALV-J, providing a perfect prospect for the ALV-J eradication in chinken in the future.