CAJ | 학술논문

针对氧化亚铁硫杆菌生物(T. f)淋滤法处理重金属污染底泥进行实验研究。采用分批摇床培养方法，分析生物淋滤过程中Fe2+浓度对底泥酸化、微生物生长、Fe2+氧化以及底泥中重金属溶出率的影响；借助Monod方程得到不同Fe2+浓度下氧化亚铁硫杆菌的最大比生长速率及饱和常数；通过经验方程推导出不同Fe2+浓度下重金属离子Cu2+和Zn2+的溶出速率常数。结果表明，当Fe2+浓度由2.0 g/L增加到10.0 g/L时，氧化亚铁硫杆菌的最大比生长速率由0.126 h-1上升到0.159 h-1，饱和常数由0.881 g/L下降到0.327 g/L，重金属的溶出速率常数与Fe2+浓度呈正相关；当Fe2+浓度超过10.0 g/L时，氧化亚铁硫杆菌的最大比生长速率降低，而饱和常数增大。随着Fe2+浓度的增加Fe2+氧化速率增大。综合各个动力学参数可知，Fe2+浓度为10.0 g/L是生物淋滤体系的最佳底物浓度。
침대양화아철류간균생물(T. f)림려법처리중금속오염저니진행실험연구。채용분비요상배양방법，분석생물림려과정중Fe2+농도대저니산화、미생물생장、Fe2+양화이급저니중중금속용출솔적영향；차조Monod방정득도불동Fe2+농도하양화아철류간균적최대비생장속솔급포화상수；통과경험방정추도출불동Fe2+농도하중금속리자Cu2+화Zn2+적용출속솔상수。결과표명，당Fe2+농도유2.0 g/L증가도10.0 g/L시，양화아철류간균적최대비생장속솔유0.126 h-1상승도0.159 h-1，포화상수유0.881 g/L하강도0.327 g/L，중금속적용출속솔상수여Fe2+농도정정상관；당Fe2+농도초과10.0 g/L시，양화아철류간균적최대비생장속솔강저，이포화상수증대。수착Fe2+농도적증가Fe2+양화속솔증대。종합각개동역학삼수가지，Fe2+농도위10.0 g/L시생물림려체계적최가저물농도。
The bioleaching kinetics of heavy metal from contaminated sediment was studied by batch experiments with Thiobacillus ferrooxidans (T. f). The effects of Fe2+concentrations on the sediment acidification, the growth of T. f, Fe2+oxidation, and solubilization ratio of heavy metal during the bioleaching process were investigated. Monod equation was used to derive the maximum specific growth rate and the saturation constant of the T. f with different Fe2+concentrations. An empirical equation was also used to derive the rate constants of heavy metal ions (Cu2+ and Zn2+) solubilization with different Fe2+concentrations. The results show that when Fe2+concentration increases from 2.0 g/L to 10.0 g/L the maximum specific growth rate of T. f increases from 0.129 h-1 to 0.159 h-1, the saturation constant reduces from 0.881 g/L to 0.327 g/L and the rate constant of heavy metals solubilization is linearly dependent on the Fe2+concentrations. When Fe2+concentrations is over 10.0 g/L, the maximum specific growth rate of the T. f reduces, while the saturation constant increases. The Fe2+oxidation rates increase with increasing the Fe2+concentrations. Taking all the kinetics parameters into account, the optimal concentration of Fe2+is found to be 10.0 g/L.