中国调味品
中國調味品
중국조미품
CHINESE CONDIMENT
2014年
5期
53-58
,共6页
张竺英%刘昭明%黄翠姬%时粲%徐财凤%陆金池
張竺英%劉昭明%黃翠姬%時粲%徐財鳳%陸金池
장축영%류소명%황취희%시찬%서재봉%륙금지
γ-氨基丁酸%乳酸菌%筛选%鉴定
γ-氨基丁痠%乳痠菌%篩選%鑒定
γ-안기정산%유산균%사선%감정
γ-aminobutyric acid%lactic acid bacteria%screening%identification
为从豆豉中获得产γ-氨基丁酸的乳酸菌,采用高效液相色谱法对从广西黄姚豆豉中分离筛选得到的16株乳酸菌的产γ-氨基丁酸能力进行了研究。结果表明:菌株HY15的产γ-氨基丁酸能力较好。该菌株在含1%(W/W)谷氨酸钠的MRS发酵培养基中37℃厌氧培养48 h 后,发酵液中γ-氨基丁酸产量达到0.161 g/L。经形态学特征、生理生化试验和16S rDNA基因鉴定,确定菌株 HY15为戊糖片球菌(Pediococcus pentosaceus)。
為從豆豉中穫得產γ-氨基丁痠的乳痠菌,採用高效液相色譜法對從廣西黃姚豆豉中分離篩選得到的16株乳痠菌的產γ-氨基丁痠能力進行瞭研究。結果錶明:菌株HY15的產γ-氨基丁痠能力較好。該菌株在含1%(W/W)穀氨痠鈉的MRS髮酵培養基中37℃厭氧培養48 h 後,髮酵液中γ-氨基丁痠產量達到0.161 g/L。經形態學特徵、生理生化試驗和16S rDNA基因鑒定,確定菌株 HY15為戊糖片毬菌(Pediococcus pentosaceus)。
위종두시중획득산γ-안기정산적유산균,채용고효액상색보법대종엄서황요두시중분리사선득도적16주유산균적산γ-안기정산능력진행료연구。결과표명:균주HY15적산γ-안기정산능력교호。해균주재함1%(W/W)곡안산납적MRS발효배양기중37℃염양배양48 h 후,발효액중γ-안기정산산량체도0.161 g/L。경형태학특정、생리생화시험화16S rDNA기인감정,학정균주 HY15위무당편구균(Pediococcus pentosaceus)。
In order to obtainγ-aminobutyric acid from lactic acid bacteria,the ability ofγ-aminobutyric acid production of the 16 strains of lactic acid bacteria isolated from Huangyao Douchi,Guangxi Prov-ince are investigated by using High Performance Liquid Chromatography.The results show that the strain HY15 has better producing ability ofγ-aminobutyric acid.After 48 h of inoculation in MRS me-dium containing 1% (W/W)monosodium glutamate,it can produceγ-aminobutyric acid with the con-centration of 0.161 g/L.Based on morphological characteristics,physiological and biochemical tests, and 16S rDNA sequence analysis,the strain HY15 is identified as Pediococcus pentosaceus.