中国调味品
中國調味品
중국조미품
CHINESE CONDIMENT
2014年
5期
45-48
,共4页
马伟玲%尹礼国%张文学%陈功%李双
馬偉玲%尹禮國%張文學%陳功%李雙
마위령%윤례국%장문학%진공%리쌍
泡菜%乳酸菌%发酵%增殖培养基
泡菜%乳痠菌%髮酵%增殖培養基
포채%유산균%발효%증식배양기
pickles%lactic acid bacteria%fermentation%proliferation medium
泡菜乳酸菌LJ-4具有良好的产酸特性,是一株可开发为泡菜发酵直投菌剂的乳酸菌。为促进其增殖培养,采用单因素和正交实验对其增殖培养基的碳源、氮源、生长因子进行了优化。研究结果表明最优增殖培养基组成为:1.5%果糖,1.5%胰蛋白胨,7%玉米汁,10%青椒汁,30%番茄汁,15%豆芽汁。泡菜乳酸菌LJ-4在优化的增殖培养基中,37℃培养16 h,菌体浓度[lg(cfu/mL)]可达到10.11。
泡菜乳痠菌LJ-4具有良好的產痠特性,是一株可開髮為泡菜髮酵直投菌劑的乳痠菌。為促進其增殖培養,採用單因素和正交實驗對其增殖培養基的碳源、氮源、生長因子進行瞭優化。研究結果錶明最優增殖培養基組成為:1.5%果糖,1.5%胰蛋白胨,7%玉米汁,10%青椒汁,30%番茄汁,15%豆芽汁。泡菜乳痠菌LJ-4在優化的增殖培養基中,37℃培養16 h,菌體濃度[lg(cfu/mL)]可達到10.11。
포채유산균LJ-4구유량호적산산특성,시일주가개발위포채발효직투균제적유산균。위촉진기증식배양,채용단인소화정교실험대기증식배양기적탄원、담원、생장인자진행료우화。연구결과표명최우증식배양기조성위:1.5%과당,1.5%이단백동,7%옥미즙,10%청초즙,30%번가즙,15%두아즙。포채유산균LJ-4재우화적증식배양기중,37℃배양16 h,균체농도[lg(cfu/mL)]가체도10.11。
Lactic acid bacteria LJ-4 isolated from pickles has good acid production characteristics;it could be used for the development of kimchi fermentation DVS agent.In order to promote its prolifer-ation,the single factor and orthogonal experiments are used to optimize the carbon source,nitrogen source and growth factor in the proliferation medium.The results indicate that the most suitable me-dium for Lactobacillus namurensis growth is consisted of 1 .5% fructose,1 .5% tryptone,7% corn syrup,10% green pepper sauce,30% tomato juice and 15% bean sprouts juice.The number of living Lactobacillus namurensis cells is over 1010 cfu/mL after 16 h of cultivation in optimized medium at 37 ℃.