广西红曲米中产Monacolin K红曲霉的分离及鉴定
엄서홍곡미중산Monacolin K홍곡매적분리급감정
Isolation and Identification of Monascus sp. Strain with Monacolin K Producing Ability from Guangxi Red Yeast Rice
  • 万方数据
    中国调味品 중국조미품
    CHINESE CONDIMENT
    2014年 5期 36-40 ,共5页

    黄翠姬%吴兰%卢莉%张竺英%刘昭明

    황취희%오란%로리%장축영%류소명

    红曲霉%分离与鉴定%18S rDNA%28S rDNA D1/D2 紅麯黴%分離與鑒定%18S rDNA%28S rDNA D1/D2
    홍곡매%분리여감정%18S rDNA%28S rDNA D1/D2
    Monacolin K%isolation and identification%18S rDNA%28S rDNA D1/D2
    研究了从广西红曲米中分离纯化得到的6株红曲霉菌株产 Monacolin K 的能力并对高产菌株进行鉴定。结果表明：6株红曲霉均具有产Monacolin K的能力，其中LH-M05发酵液中Monacolin K含量最高，达到了177．93 mg/L。依据形态特征、生理生化特征和18S rDNA和28S rDNA D1/D2区序列将菌株LH-M05确定为紫色红曲霉（Monascus purpureus）。
    연구료종엄서홍곡미중분리순화득도적6주홍곡매균주산 Monacolin K 적능력병대고산균주진행감정。결과표명：6주홍곡매균구유산Monacolin K적능력，기중LH-M05발효액중Monacolin K함량최고，체도료177．93 mg/L。의거형태특정、생리생화특정화18S rDNA화28S rDNA D1/D2구서렬장균주LH-M05학정위자색홍곡매（Monascus purpureus）。
    Six strains of Monascus spp.are isolated from red yeast rice in Guangxi Province.The strains with high Monacolin K producing ability are identified.The results show that Monacolin K producing ability is observed for all six strains tested.Among them,LH-M05 strain gives concentra-tion of 177.93 mg/L in fermentation broth.Based on morphological,physiological test and phyloge-netic analysis of 18S rDNA and 28S rDNA D1/D2 sequence,LH-M05 strain is identified as Monascus purpureus.
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