CAJ | 학술논문

为了研究大肠杆菌L型，以氨苄青霉素作为诱导剂，采用药物纸片法对细菌进行反复传代，革兰氏染色及细胞壁染色、镜检，观察菌落及菌体形态，对稳定的L型菌进行致病性及药物敏感性试验。结果表明，大肠杆菌经11次传代可形成稳定的L型菌，且该L菌具有致病性；L型菌对氨苄西林、头孢他啶、头孢唑啉具有一定的耐药性。本研究为开展L型菌检测及指导临床用药提供理论依据。
위료연구대장간균L형，이안변청매소작위유도제，채용약물지편법대세균진행반복전대，혁란씨염색급세포벽염색、경검，관찰균락급균체형태，대은정적L형균진행치병성급약물민감성시험。결과표명，대장간균경11차전대가형성은정적L형균，차해L균구유치병성；L형균대안변서림、두포타정、두포서람구유일정적내약성。본연구위개전L형균검측급지도림상용약제공이론의거。
E.coli were repeatedly passage by disk diffusion method with ampicillin as inducer to study the L-form. E.coli L-form were identified with gram stain, tested under microscope and colony observation and thallus observation. Pathogenicity and drug susceptibility test were also carried out. Results showed that E.coli can emerge stabile pathogenic L-form after 11times subculture and appear resistant to Ampicillin , Ceftazidime and Cefazolin. The results provided theoretical basis for clinical treatment and detection.