应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2014年
5期
957-961
,共5页
杨红梅%王永生%黎俊宏%欧阳运富
楊紅梅%王永生%黎俊宏%歐暘運富
양홍매%왕영생%려준굉%구양운부
反相高效液相色谱%多环芳烃%尿
反相高效液相色譜%多環芳烴%尿
반상고효액상색보%다배방경%뇨
reversed phase high performance liquid chromatography(RP-HPLC)%polycyclic aromatic hydrocarbons(PAHs)%urinary
建立了用反相高效液相色谱-紫外检测法同时检测人尿中6种多环芳烃羟基代谢产物的新方法。采用 Dia-monsil TM C18(5μm,150 nm ×4.6 nm)色谱柱分离,以 V(甲醇): V(乙酸钠缓冲液)=66:34为流动相,在波长280 nm检测,流速为0.95 mL/ min。结果表明,当9-羟基菲、α-萘酚、β-萘酚、1-羟基芘、2-羟基芴和9-羟基芴的摩尔浓度分别为0.105~500.0μmol/ L,0.106~135.0μmol/ L,0.120~135.0μmol/ L,0.085~50.00μmol/ L,0.094~200.0μmol/ L 和0.101~200.0μmol/ L 时,峰面积与质量浓度呈很好的线性关系,相对标准偏差分别为2.5%~4.4%,2.1%~4.3%,2.3%~3.2%,1.7%~3.9%,1.9%~2.6%和1.8%~3.5%(n =6),平均回收率分别为92.6%,100.4%,101.0%,91.0%,106.7%和95.5%。方法准确可靠,用于尿样测定,结果满意。
建立瞭用反相高效液相色譜-紫外檢測法同時檢測人尿中6種多環芳烴羥基代謝產物的新方法。採用 Dia-monsil TM C18(5μm,150 nm ×4.6 nm)色譜柱分離,以 V(甲醇): V(乙痠鈉緩遲液)=66:34為流動相,在波長280 nm檢測,流速為0.95 mL/ min。結果錶明,噹9-羥基菲、α-萘酚、β-萘酚、1-羥基芘、2-羥基芴和9-羥基芴的摩爾濃度分彆為0.105~500.0μmol/ L,0.106~135.0μmol/ L,0.120~135.0μmol/ L,0.085~50.00μmol/ L,0.094~200.0μmol/ L 和0.101~200.0μmol/ L 時,峰麵積與質量濃度呈很好的線性關繫,相對標準偏差分彆為2.5%~4.4%,2.1%~4.3%,2.3%~3.2%,1.7%~3.9%,1.9%~2.6%和1.8%~3.5%(n =6),平均迴收率分彆為92.6%,100.4%,101.0%,91.0%,106.7%和95.5%。方法準確可靠,用于尿樣測定,結果滿意。
건립료용반상고효액상색보-자외검측법동시검측인뇨중6충다배방경간기대사산물적신방법。채용 Dia-monsil TM C18(5μm,150 nm ×4.6 nm)색보주분리,이 V(갑순): V(을산납완충액)=66:34위류동상,재파장280 nm검측,류속위0.95 mL/ min。결과표명,당9-간기비、α-내분、β-내분、1-간기비、2-간기물화9-간기물적마이농도분별위0.105~500.0μmol/ L,0.106~135.0μmol/ L,0.120~135.0μmol/ L,0.085~50.00μmol/ L,0.094~200.0μmol/ L 화0.101~200.0μmol/ L 시,봉면적여질량농도정흔호적선성관계,상대표준편차분별위2.5%~4.4%,2.1%~4.3%,2.3%~3.2%,1.7%~3.9%,1.9%~2.6%화1.8%~3.5%(n =6),평균회수솔분별위92.6%,100.4%,101.0%,91.0%,106.7%화95.5%。방법준학가고,용우뇨양측정,결과만의。
A novel method for assaying major metabolites of 6 kinds of polycyclic aromatic hydrocarbons in human urine by RP-HPLC-UV were established. These were detected at 280 nm and separated on Dia-monsil TM C18(5 μm,150 nm × 4. 6 nm)by using sodium acetate buffer solution(66: 34,V: V)as mobile phase when the flow rate was 0. 95 mL/ min. The result showed that the calibration curves of 9-hydroxy-phenanthrene,α-naphthol,β-naphthol,1-hydroxypyrene,2-hydroxyfluorene and 9-hydroxyfluorene showed good linearities in the ranges of 0. 105 ~ 500. 0 μmol/ L,0. 106 ~ 135. 0 μmol/ L,0. 120 ~135. 0 μmol/ L,0. 085 ~ 50. 0 μmol/ L,0. 094 ~ 200. 0 μmol/ L and 0. 101 ~ 200. 0 μmol/ L,respective-ly. The relative standard deviations(RSD)were 2. 5% ~ 4. 4% ,2. 1% ~ 4. 3% ,2. 3% ~ 3. 2% ,1. 7% ~3. 9% ,1. 9% ~ 2. 6% ,1. 8% ~ 3. 5%( n = 6)and the average recoveries were 92. 6% ,100. 4% , 101. 0% ,91. 0% ,106. 7% and 95. 5% ,respectively. The proposed method was accurate and suitable for the identification and quantification of these compositions in human urine.