CAJ | 학술논문

为了优化蚕沙中黄酮和生物碱的提取工艺，通过单因素试验和正交试验相结合的方法，比较了不同提取方法、提取溶剂、提取时间、提取温度等条件。结果表明：盐酸浓度对生物碱提取率有显著影响，提取温度和提取时间对生物碱提取率无显著影响；各因素对黄酮提取率均无显著影响。综合考虑，最佳生物碱和黄酮提取工艺条件为：0．01 mol／L盐酸，90℃水浴提取2次，每次1h。该提取工艺稳定可靠，可用于蚕沙黄酮和生物碱的提取。
위료우화잠사중황동화생물감적제취공예，통과단인소시험화정교시험상결합적방법，비교료불동제취방법、제취용제、제취시간、제취온도등조건。결과표명：염산농도대생물감제취솔유현저영향，제취온도화제취시간대생물감제취솔무현저영향；각인소대황동제취솔균무현저영향。종합고필，최가생물감화황동제취공예조건위：0．01 mol／L염산，90℃수욕제취2차，매차1h。해제취공예은정가고，가용우잠사황동화생물감적제취。
In order to optimize the extraction technology of flavonoids and alkaloids from silkworm faeces , we studied various factors affecting the extraction through single -factor test and orthogonal test .The results showed that hydrochloric concentration had a significant effect on the extraction efficiency of alkaloids , but extraction temperature and extraction time had no significant effect on it.All factors had no significant effect on the extraction efficiency of flavonoids .The optimum extraction technology of flavonoids and alkaloids from silkworm faeces was extracting with 0.01 mol/L hydrochloric acid through 90 ℃ water bath for 2 times and 1 hour each time.This extraction technology was efficient and reliable , and it could be used for the extraction of flavonoids and alkaloids from silkworm faeces .