江西农业学报
江西農業學報
강서농업학보
ACTA AGRICULTURAE JIANGXI
2014年
5期
42-46
,共5页
马存强%李静%周斌星%柴洁%杨超%任小盈%张梦华
馬存彊%李靜%週斌星%柴潔%楊超%任小盈%張夢華
마존강%리정%주빈성%시길%양초%임소영%장몽화
普洱茶%发酵%黑曲霉%咖啡碱代谢
普洱茶%髮酵%黑麯黴%咖啡堿代謝
보이다%발효%흑곡매%가배감대사
Pu-erh tea%Fermentation%Aspergillus niger%Caffeine metabolism
普洱茶固态发酵过程的实质是微生物代谢作用,而黑曲霉在代谢中为优势菌,对咖啡碱代谢产生巨大影响。以黑曲霉为单一菌种,分别接种到茶叶、茶汤和含咖啡碱的液态培养基,并测定不同发酵阶段嘌呤碱的含量。结果表明,黑曲霉对茶叶碱的影响大于可可碱,对可可碱的影响大于咖啡碱,在咖啡碱液态培养基发酵中,茶叶碱增加幅度最大,且与咖啡碱浓度存在正相关性。
普洱茶固態髮酵過程的實質是微生物代謝作用,而黑麯黴在代謝中為優勢菌,對咖啡堿代謝產生巨大影響。以黑麯黴為單一菌種,分彆接種到茶葉、茶湯和含咖啡堿的液態培養基,併測定不同髮酵階段嘌呤堿的含量。結果錶明,黑麯黴對茶葉堿的影響大于可可堿,對可可堿的影響大于咖啡堿,在咖啡堿液態培養基髮酵中,茶葉堿增加幅度最大,且與咖啡堿濃度存在正相關性。
보이다고태발효과정적실질시미생물대사작용,이흑곡매재대사중위우세균,대가배감대사산생거대영향。이흑곡매위단일균충,분별접충도다협、다탕화함가배감적액태배양기,병측정불동발효계단표령감적함량。결과표명,흑곡매대다협감적영향대우가가감,대가가감적영향대우가배감,재가배감액태배양기발효중,다협감증가폭도최대,차여가배감농도존재정상관성。
The essence of pu-erh tea solid-fermentation process is microbial metabolism , and Aspergillus niger is a dominant fungus in the metabolism and has a huge impact on caffeine metabolism .In this study, Aspergillus niger strains were inoculated into tea, tea infusion, and liquid medium containing caffeine , then the content of purine base at different stages of fermentation was de -termined .The results showed that Aspergillus niger had grater effect on theophylline than on theobromine , and greater effect on theo-bromine than on caffeine .In addition , during the liquid -fermentation of pure caffeine , theophylline content increased to a maxi-mum extent , and it was positively correlated with caffeine concentration .