CAJ | 학술논문

以结球甘蓝种子为试材，采用正交试验和均匀试验方法，研究了磁场强度、作用时间及处理前种子浸泡时间对各发芽指标的影响以及各影响因素的主次顺序，并对磁场参数中磁场强度和作用时间进行大范围筛选，更进一步从可溶性蛋白含量、丙二醛含量、抗氧化酶活性等方面探讨磁场处理提高种子活力的作用机理。结果表明：适宜的磁场处理可以促进种子萌发，提高种子活力，以20℃蒸馏水浸泡8 h 后1000～3500 GS 磁场处理1～6 min 为宜，但长时间、大强度磁场处理反而抑制种子萌发；磁场强度为种子萌发的主要影响因子，作用时间和浸泡时间的主次顺序因发芽指标的不同而不同；与对照相比，磁场处理的种子萌发期间可溶性蛋白含量降低或升高的速率、MDA 含量下降的幅度和速率、3种抗氧化酶活性上升的速率均加快，从而提高种子活力。
이결구감람충자위시재，채용정교시험화균균시험방법，연구료자장강도、작용시간급처리전충자침포시간대각발아지표적영향이급각영향인소적주차순서，병대자장삼수중자장강도화작용시간진행대범위사선，경진일보종가용성단백함량、병이철함량、항양화매활성등방면탐토자장처리제고충자활력적작용궤리。결과표명：괄의적자장처리가이촉진충자맹발，제고충자활력，이20℃증류수침포8 h 후1000～3500 GS 자장처리1～6 min 위의，단장시간、대강도자장처리반이억제충자맹발；자장강도위충자맹발적주요영향인자，작용시간화침포시간적주차순서인발아지표적불동이불동；여대조상비，자장처리적충자맹발기간가용성단백함량강저혹승고적속솔、MDA 함량하강적폭도화속솔、3충항양화매활성상승적속솔균가쾌，종이제고충자활력。
Taking Brassica oleracea L. var. capitata L. seed as experimental material,the effect of magnetic field intensity,and magnetic acting time and seed soaking time before treatment on seed germination index and primary and secondary relations between all factors were studied by orthogonal test and uniform test method.Then the large scale screening of magnetic field intensity and magnetic acting time were carried out to further investigate the mechanism of using magnetic field treatment to improve seed vigor from the view points of soluble protein content,malondialdehyde content,and antioxidant enzyme activity.The results indicated that suitable magnetic field treatment could promote seed germination and improve seed vigor.Magnetic field intensity was the main factor affecting seed germination.The primary and secondary order of action time and soaking time were varied with different germination indexes. Seed germination was inhibited by too high magnetic field intensity for a long time. Soaking seed in 20 ℃ distilled water for 8 hours,1-6 minutes was appropriate treatment by 1 000-3 500 GS magnetic field.After magnetic field treatment,the soluble sugar and protein contents were increased,MDA content decreased,and enzyme activities of antioxidant system were increased,thus the seed germination effect was improved.