CAJ | 학술논문

利用形态学标记、SSR 分子标记技术对市场上广泛种植的甜瓜品种进行分类鉴定。利用形态学聚类分析，得到60份材料间的相似系数为0.73～0.94，在相似系数为0.74处，60份甜瓜材料被分为2类，为薄皮甜瓜和厚皮甜瓜；在相似系数为0.77处，薄皮甜瓜按照单果质量、熟性、果皮底色、芳香气分为4组，实现初步分类。经过 SSR 标记聚类分析，得到60份材料间的相似系数为0.75～0.95，当相似系数为0.77时，可以将所有供试材料分为6类。利用 SSR 分子标记技术，为60份不同甜瓜品种构建唯一的指纹图谱。18对 SSR 引物，每对引物可以检测到4～11条数目不等的多态性条带，平均为8条。多态性信息含量（PIC）平均为0.71，变化范围为0.58～0.88。采用 QR 编码为60份甜瓜材料构建了唯一的指纹图谱。试验结果表明形态学标记对60份材料实现初步分类，SSR 分子标记则能够有效地区别60份材料，并且为建立甜瓜品种的核酸指纹图谱库提供了理论数据，实现对甜瓜品种进行快速、准确的鉴定。
이용형태학표기、SSR 분자표기기술대시장상엄범충식적첨과품충진행분류감정。이용형태학취류분석，득도60빈재료간적상사계수위0.73～0.94，재상사계수위0.74처，60빈첨과재료피분위2류，위박피첨과화후피첨과；재상사계수위0.77처，박피첨과안조단과질량、숙성、과피저색、방향기분위4조，실현초보분류。경과 SSR 표기취류분석，득도60빈재료간적상사계수위0.75～0.95，당상사계수위0.77시，가이장소유공시재료분위6류。이용 SSR 분자표기기술，위60빈불동첨과품충구건유일적지문도보。18대 SSR 인물，매대인물가이검측도4～11조수목불등적다태성조대，평균위8조。다태성신식함량（PIC）평균위0.71，변화범위위0.58～0.88。채용 QR 편마위60빈첨과재료구건료유일적지문도보。시험결과표명형태학표기대60빈재료실현초보분류，SSR 분자표기칙능구유효지구별60빈재료，병차위건립첨과품충적핵산지문도보고제공료이론수거，실현대첨과품충진행쾌속、준학적감정。
Studies on classification and identification of 60 melon(Cucumis melo L.)materials were conducted by SSR marker and Morphological marker. Based on morphological cluster analysis the genetic similarity coefficient of the 60 materials ranged from 0.73-0.94,and at the genetic similarity coefficient of 0.74 these melons could be classified into 2 categories:Cucumis melo ssp. conomon and C. melo ssp. melo. Cucumis melo ssp. conomon could be subdivided into 4 groups,which are preliminarily classified by single fruit weight,peel color,aroma,maturity. The result of SSR cluster analysis showed that the genetic similarity coefficient of the 60 accessions ranged from 0.75-0.95. Also,the test materials could be divided into 6 categories at the genetic similarity coefficient of 0.77. 18 pairs of the SSR primers could detect 4-11 polymorphic bands with an average of 8 bands. The average polymorphism information content(PIC)was 0.71 with the changing range of 0.58-0.88. The fingerprinting effectively distinguished the 60 materials and every accession had unique fingerprinting using QR code.