食品研究与开发
食品研究與開髮
식품연구여개발
FOOD RESEARCH AND CEVELOPMENT
2014年
8期
107-110
,共4页
马卫俊%延慧君%宋新波%刘岱琳
馬衛俊%延慧君%宋新波%劉岱琳
마위준%연혜군%송신파%류대림
根皮苷%反相-高效液相色谱法%微生物转化%底物消耗率
根皮苷%反相-高效液相色譜法%微生物轉化%底物消耗率
근피감%반상-고효액상색보법%미생물전화%저물소모솔
phlorhizin%RP-HPLC%biotransformation%consumption rate
利用反相高效液相法测定24种真菌对根皮苷的转化产物中根皮苷的含量，并对有转化能力的真菌菌种进行底物消耗率的测定。RP-HPLC色谱条件：Phenomenex Luna C18色谱柱（250 mm×4.6 mm，5μm）；流动相A相：冰醋酸：水=1∶99（V/V），B相∶乙腈∶水∶冰醋酸=70∶29.8∶0.2（体积比），进行梯度洗脱：0（0%B）-20（25%B）-50（35%B）-80（60%B）-85 min（0%B）；流速：1.0 mL/min；检测波长：280 nm。根皮苷质量浓度在0.09840 mg/mL～0.7872 mg/mL内线性关系良好，回归方程为Y=4×107X+44514（R2=0.999）。经HPLC检测，3种真菌对根皮苷有转化作用，当培养基中根皮苷质量浓度为0.3 mg/mL时，测得培养后底物消耗率分别为45.31%，59.01%，55.60%。结果表明RP-HPLC法方法简单准确、重现性好，可适用于根皮苷微生物转化研究中非单一转化产物的定性和定量分析。
利用反相高效液相法測定24種真菌對根皮苷的轉化產物中根皮苷的含量，併對有轉化能力的真菌菌種進行底物消耗率的測定。RP-HPLC色譜條件：Phenomenex Luna C18色譜柱（250 mm×4.6 mm，5μm）；流動相A相：冰醋痠：水=1∶99（V/V），B相∶乙腈∶水∶冰醋痠=70∶29.8∶0.2（體積比），進行梯度洗脫：0（0%B）-20（25%B）-50（35%B）-80（60%B）-85 min（0%B）；流速：1.0 mL/min；檢測波長：280 nm。根皮苷質量濃度在0.09840 mg/mL～0.7872 mg/mL內線性關繫良好，迴歸方程為Y=4×107X+44514（R2=0.999）。經HPLC檢測，3種真菌對根皮苷有轉化作用，噹培養基中根皮苷質量濃度為0.3 mg/mL時，測得培養後底物消耗率分彆為45.31%，59.01%，55.60%。結果錶明RP-HPLC法方法簡單準確、重現性好，可適用于根皮苷微生物轉化研究中非單一轉化產物的定性和定量分析。
이용반상고효액상법측정24충진균대근피감적전화산물중근피감적함량，병대유전화능력적진균균충진행저물소모솔적측정。RP-HPLC색보조건：Phenomenex Luna C18색보주（250 mm×4.6 mm，5μm）；류동상A상：빙작산：수=1∶99（V/V），B상∶을정∶수∶빙작산=70∶29.8∶0.2（체적비），진행제도세탈：0（0%B）-20（25%B）-50（35%B）-80（60%B）-85 min（0%B）；류속：1.0 mL/min；검측파장：280 nm。근피감질량농도재0.09840 mg/mL～0.7872 mg/mL내선성관계량호，회귀방정위Y=4×107X+44514（R2=0.999）。경HPLC검측，3충진균대근피감유전화작용，당배양기중근피감질량농도위0.3 mg/mL시，측득배양후저물소모솔분별위45.31%，59.01%，55.60%。결과표명RP-HPLC법방법간단준학、중현성호，가괄용우근피감미생물전화연구중비단일전화산물적정성화정량분석。
To study 24 species of fungi on the conversion of Phlorhizin and determine the consumption rate of activated fungi by RP-HPLC. The microbial transformation reactions of Phlorhizin were identified by reversed-phase high-performance liquid chromatography (RP-HPLC). The optimum HPLC conditions were as follows:column was Phenomenex Luna C18 (250 mm×4.6 mm, 5μm). Gradient elution with mobile phase A:HAC:H2O=1∶99(V/V) and B:MeCN∶H2O∶HAC=70∶29.8∶0.2(V/V/V) in the program of 0(0%B)-20(25%B)-50 (35%B)-80(60%B)-85 min(0%B) at a flow rate of 1.0 mL/min was utilized after optimization. The detection wavelength was set at 280 nm. The calibration curve was Y=4×107X+44 514 (R2=0.999) with the linear in the range of 0.098 40 mg/mL-0.787 2 mg/mL for Phlorhizin. The results showed that Phlorhizin was biotransformed by three kinds of fungi and the conversion rate were 45.31%, 59.01%, 55. 60%when Phlorhizin concentration in medium was 0.3 mg/mL. The results indicated that this detective method was stable and accurate for qualitation and quantitation of the biotransformation products of Phlorhizin.
