CAJ | 학술논문

测定干酪乳杆菌（Lactobacillus casei）LC2W和植物乳杆菌（Lactobacillus plantarum）ST-III在以非消化性寡糖为唯一碳源的MRS液体培养液中的吸光值，以及在添加非消化性寡糖的脱脂牛乳中发酵后，发酵乳中益生菌菌落总数、乳酸含量和pH，研究非消化性寡糖对L. casei LC2W和L. plantarum ST-III体外增殖的影响。结果显示，低聚半乳糖和菊粉可以明显促进L. casei LC2W的体外增殖（P＜0.01），其最佳添加浓度为3%；而对L. plantarum ST-III体外增殖促进作用最显著的是棉子糖和菊粉（P＜0.01），最佳添加浓度为2%。研究表明不同益生菌菌株对非消化性寡糖发挥体外增殖效应具有选择性，研究结果可为益生菌产品的开发提供技术基础。
측정간락유간균（Lactobacillus casei）LC2W화식물유간균（Lactobacillus plantarum）ST-III재이비소화성과당위유일탄원적MRS액체배양액중적흡광치，이급재첨가비소화성과당적탈지우유중발효후，발효유중익생균균락총수、유산함량화pH，연구비소화성과당대L. casei LC2W화L. plantarum ST-III체외증식적영향。결과현시，저취반유당화국분가이명현촉진L. casei LC2W적체외증식（P＜0.01），기최가첨가농도위3%；이대L. plantarum ST-III체외증식촉진작용최현저적시면자당화국분（P＜0.01），최가첨가농도위2%。연구표명불동익생균균주대비소화성과당발휘체외증식효응구유선택성，연구결과가위익생균산품적개발제공기술기출。
In order to evaluate the effects of nondigestible oligosaccharides (NDOs) on the in vitro growth of two probiotic strains, L. casei LC2W and L. plantarum ST-III, the absorption values of MRS cultures at 600 nm with NDOs as the unique carbon source were measured, and the colony count, lactic acid content, and pH value of fermented skim milk with the addition of NDOs were detected in this paper. The results showed that galacto-oligosaccharide (GOS) and inulin could promote the proliferation of L. casei LC2W significantly (P<0.01), and the optimal content of GOS and inulin was 3%. Raffinose and inulin had a most remarkable influence (P<0.01) on the growth of L. plantarum ST-III, and the optimal concentration of additive was 2%. The research indicated that NDOs have selectivity on the in vitro proliferation of probiotic strains. The results can provide technical basis of the research and development of probiotic products.