CAJ | 학술논문

采用30%的乙腈水（体积分数）为稀释液，建立了样品直接稀释、高效液相色谱（HPLC）分离、示差折光检测器（RID）快速测定葡萄酒中的甘油、果糖、葡萄糖、蔗糖的可靠方法。该方法以保留时间（Rt）定性，外标法定量；在0.05 mg/mL~5 mg/mL的浓度范围内，标准曲线有良好的线性关系，相关系数大于0.9995；样品在0.25、2.5、25.0 g/L 3个加标水平内的回收率范围为96%~102%（n=10），相对标准偏差小于8.0%；方法的定性检出限（LOD）为0.04 g/L、定量检出限（LOQ）为0.10 g/L。
채용30%적을정수（체적분수）위희석액，건립료양품직접희석、고효액상색보（HPLC）분리、시차절광검측기（RID）쾌속측정포도주중적감유、과당、포도당、자당적가고방법。해방법이보류시간（Rt）정성，외표법정량；재0.05 mg/mL~5 mg/mL적농도범위내，표준곡선유량호적선성관계，상관계수대우0.9995；양품재0.25、2.5、25.0 g/L 3개가표수평내적회수솔범위위96%~102%（n=10），상대표준편차소우8.0%；방법적정성검출한（LOD）위0.04 g/L、정량검출한（LOQ）위0.10 g/L。
Diluent for 30%acetonitrile water, there were set up some reliable methods,like dilute the sample directly, High performance liquid chromatography (HPLC), Refractive index detector (RID),which made a rapid determination of glycerin,fructose,glucose,sucrose in wine. The method took the retention time (Rt) qualitative, external standard method of quantitative;within the limits of concentration 0.05 mg/mL-5 mg/mL, standard curve had a good linear relationship, and the correlation coefficient was more than 0.999 5;in the level of 0.25 g/L,2.5 g/L,25.0 g/L,the add mark recovery rate was from 96%to 102%,the relative standard deviation was less than 8.0%, limit of detection(LOD) was 0.04 g/L,limit of quantitation(LOQ) was 0.10 g/L.