科技视界
科技視界
과기시계
Science&Technology Vision
2014年
8期
12-13
,共2页
赵铃%余春梅%王正凤%吴军
趙鈴%餘春梅%王正鳳%吳軍
조령%여춘매%왕정봉%오군
PD12890%含量测定%方法建立%高效液相色谱法
PD12890%含量測定%方法建立%高效液相色譜法
PD12890%함량측정%방법건립%고효액상색보법
PD12890%Determination%METHODS%HPLC
目的：PD12890原料药含量测定方法的建立。方法：采用高效液相色谱法，色谱柱：Agilent ZORBAX SB-C18(4.6×150mm，5μm)；流动相：乙腈-甲醇-水(20：50：30)；检测波长293nm；柱温：30℃；流速为1.0ml/min。结果：PD12890在0.385-0.660mg/mL范围内峰面积有良好的线性关系，r=0.9998；高、中、低浓度的平均回收率分别为99.4%，99.6%和99.8%，RSD分别为0.3%，0.2%和0.3%。结论：所用方法简单快速，专属性强，重现性好，可用于PD12890原料药的含量测定。
目的：PD12890原料藥含量測定方法的建立。方法：採用高效液相色譜法，色譜柱：Agilent ZORBAX SB-C18(4.6×150mm，5μm)；流動相：乙腈-甲醇-水(20：50：30)；檢測波長293nm；柱溫：30℃；流速為1.0ml/min。結果：PD12890在0.385-0.660mg/mL範圍內峰麵積有良好的線性關繫，r=0.9998；高、中、低濃度的平均迴收率分彆為99.4%，99.6%和99.8%，RSD分彆為0.3%，0.2%和0.3%。結論：所用方法簡單快速，專屬性彊，重現性好，可用于PD12890原料藥的含量測定。
목적：PD12890원료약함량측정방법적건립。방법：채용고효액상색보법，색보주：Agilent ZORBAX SB-C18(4.6×150mm，5μm)；류동상：을정-갑순-수(20：50：30)；검측파장293nm；주온：30℃；류속위1.0ml/min。결과：PD12890재0.385-0.660mg/mL범위내봉면적유량호적선성관계，r=0.9998；고、중、저농도적평균회수솔분별위99.4%，99.6%화99.8%，RSD분별위0.3%，0.2%화0.3%。결론：소용방법간단쾌속，전속성강，중현성호，가용우PD12890원료약적함량측정。
Objective: To establish the method for the measurement of content in PD12890 by High Performance liquid chromatography (HPLC). Methods: With the HPLC method, the chromatographic column was applied Agilent ZORBAX SB-C18 (4.6í150mm,5μm); the mobile phase was applied acetonitrile, methanol and water (20:50:30); the flow rate was 1.0 mL/min; and the detected wavelength was 293 nm, the column temperature was 30℃. Results: The data showed a good linear relationship between peak area and concentration of PD12890 within 0.385-0.660 mg/mL, r=0.9998. The three concentration average recoveries were 99.4%, 99.6% and 99.8%, the RSD were 0.3%, 0.2% and 0.3%, respectively. Conclusion:The HPLC method was simple, rapid, and with strong specificity and good reproducibility which can be applied in content determination for PD12890.
