CAJ | 학술논문

采用响应面优化尿素包合法富集黑加仑籽油中γ-亚麻酸的最佳工艺。在单因素试验基础上，选择包合温度、尿素/混合脂肪酸配比、95%乙醇/尿素配比（mL/g）、包合时间为考察因素，进行四因素三水平的Box-Behnken中心组合设计，采用响应面法分析4个因素对籽油中γ-亚麻酸含量的影响。结果表明，尿素包合法富集黑加仑籽油中γ-亚麻酸的最佳工艺为包合温度-20℃、尿素/混合脂肪酸配比3.5∶1、95%乙醇/尿素配比2.5∶1（mL/g）、包合时间21 h，在此条件下，包合后籽油中γ-亚麻酸含量平均值为24.27%。
채용향응면우화뇨소포합법부집흑가륜자유중γ-아마산적최가공예。재단인소시험기출상，선택포합온도、뇨소/혼합지방산배비、95%을순/뇨소배비（mL/g）、포합시간위고찰인소，진행사인소삼수평적Box-Behnken중심조합설계，채용향응면법분석4개인소대자유중γ-아마산함량적영향。결과표명，뇨소포합법부집흑가륜자유중γ-아마산적최가공예위포합온도-20℃、뇨소/혼합지방산배비3.5∶1、95%을순/뇨소배비2.5∶1（mL/g）、포합시간21 h，재차조건하，포합후자유중γ-아마산함량평균치위24.27%。
The present study aimed to optimize enrichment ofγ-linolenic acid from blackcurrant seeds oil using urea inclusion method and response surface methodology (RSM) combined with a 4-variable, 3-level Box-Behnken central composite design (CCD) on the basis of single factor experiments. Inclusion temperature ,ratio of urea-to-fatty acid , ratio of 95%ethanol-to-urea(mL/g) and inclusion time were studied. Results showed that the optimized enrichment conditions are inclusion temperature-20℃, urea-to-fatty acids ratio 3.5∶1, 95%ethanol-to-urea ratio 2.5∶1 (mL/g) and inclusion time 21 h,resulted in a averageγ-linolenic acids of seeds oil yield of up to 24.27%.