中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
9期
1692-1696
,共5页
早老素1%高脂膳食%体重调节%EGCG
早老素1%高脂膳食%體重調節%EGCG
조로소1%고지선식%체중조절%EGCG
Presenilin-1%High-fat diet%Weight regulation%EGCG
目的：使用前脑特异性敲除早老素-1（PS-1FB-KO）的小鼠模型，观察饮食对其体重的影响以及表没食子儿茶素没食子酸酯（EGCG）对该小鼠体重调节的作用。方法将2月龄体重相近的PS-1FB-KO和同窝对照（CON）雄性小鼠各分为2组分别给予高脂和普通膳食。连续喂养8周后再将高脂喂食组各分为3组进行为期1周的以下处理：（1）恢复普通饲料；（2）继续给予高脂饲料同时腹腔注射EGCG；（3）继续给予高脂饲料同时腹腔注射PBS。同期连续监测小鼠的体重和血糖值。结果实验前8周，PS-1FB-KO高脂膳食喂养组小鼠的体重增长率小于CON组。实验第9周，PS-1FB-KO与CON高脂膳食喂养同时腹腔注射EGCG的体重减低率大于同基因型注射PBS组。PS-1FB-KO组与CON组的血糖在各时期并无显著性差异。结论 PS-1FB-KO使小鼠的体重调节功能产生障碍，并且这种差异并不是由外周代谢差异引起的。EGCG 能够降低高脂诱导肥胖小鼠的体重，并且其潜在的中枢神经保护作用，帮助PS-1FB-KO的小鼠减轻肥胖。
目的：使用前腦特異性敲除早老素-1（PS-1FB-KO）的小鼠模型，觀察飲食對其體重的影響以及錶沒食子兒茶素沒食子痠酯（EGCG）對該小鼠體重調節的作用。方法將2月齡體重相近的PS-1FB-KO和同窩對照（CON）雄性小鼠各分為2組分彆給予高脂和普通膳食。連續餵養8週後再將高脂餵食組各分為3組進行為期1週的以下處理：（1）恢複普通飼料；（2）繼續給予高脂飼料同時腹腔註射EGCG；（3）繼續給予高脂飼料同時腹腔註射PBS。同期連續鑑測小鼠的體重和血糖值。結果實驗前8週，PS-1FB-KO高脂膳食餵養組小鼠的體重增長率小于CON組。實驗第9週，PS-1FB-KO與CON高脂膳食餵養同時腹腔註射EGCG的體重減低率大于同基因型註射PBS組。PS-1FB-KO組與CON組的血糖在各時期併無顯著性差異。結論 PS-1FB-KO使小鼠的體重調節功能產生障礙，併且這種差異併不是由外週代謝差異引起的。EGCG 能夠降低高脂誘導肥胖小鼠的體重，併且其潛在的中樞神經保護作用，幫助PS-1FB-KO的小鼠減輕肥胖。
목적：사용전뇌특이성고제조로소-1（PS-1FB-KO）적소서모형，관찰음식대기체중적영향이급표몰식자인다소몰식자산지（EGCG）대해소서체중조절적작용。방법장2월령체중상근적PS-1FB-KO화동와대조（CON）웅성소서각분위2조분별급여고지화보통선식。련속위양8주후재장고지위식조각분위3조진행위기1주적이하처리：（1）회복보통사료；（2）계속급여고지사료동시복강주사EGCG；（3）계속급여고지사료동시복강주사PBS。동기련속감측소서적체중화혈당치。결과실험전8주，PS-1FB-KO고지선식위양조소서적체중증장솔소우CON조。실험제9주，PS-1FB-KO여CON고지선식위양동시복강주사EGCG적체중감저솔대우동기인형주사PBS조。PS-1FB-KO조여CON조적혈당재각시기병무현저성차이。결론 PS-1FB-KO사소서적체중조절공능산생장애，병차저충차이병불시유외주대사차이인기적。EGCG 능구강저고지유도비반소서적체중，병차기잠재적중추신경보호작용，방조PS-1FB-KO적소서감경비반。
Objective To investigate the influence of presenilin 1 forebrain knock-out (PS-1FB-KO) on weight-regulation by diet and EGCG in mice. Methods 2-month-old PS-1FB-KO and control (CON) male mice with similar weight were divided into two groups, which included the high-fat diet group and ordinary diet group. After 8 weeks feeding, the high-fat diet group were divided into three groups for 1 week treatment:(1)restoration to ordinary diet;(2)high-fat diet and intraperitoneal injection of EGCG; (3)high-fat diet and intraperitoneal injection of PBS. Body weight and blood glucose value were monitored over the period. Results The weight growth rate in PS-1FB-KO high-fat diet group was lower than that in the CON group after 8 weeks diet feeding. The weight loss rate in PS-1FB-KO and CON high-fat diet and intraperitoneal injection of EGCG group was less than that in PBS group. There was no significant difference in blood glucose over each period among the PS-1FB-KO and CON groups. Conclusions Disorder of weight regulation was observed in PS-1FB-KO mice, and this abnormality was not caused by differences in the peripheral metabolism. EGCG can reduce the weight of obese PS-1FB-KO mice induced by high-fat, which may be attributed to its potential central nervous protective effect.
