中华临床医师杂志（电子版）
中華臨床醫師雜誌（電子版）
중화림상의사잡지（전자판）
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
9期
1674-1678
,共5页
乳腺肿瘤%基质金属蛋白酶类%二氢杨梅素%肺转移
乳腺腫瘤%基質金屬蛋白酶類%二氫楊梅素%肺轉移
유선종류%기질금속단백매류%이경양매소%폐전이
Breast neoplasms%Matrix metalloproteinases%Dihydromyricetin%Pulmonary metastasis
目的：为了探讨二氢杨梅素的抗转移作用，在Balb/c雌性小鼠右后腿皮下建立了4T1乳腺癌移植模型，然后给予二氢杨梅素灌胃给药。方法 MTT法检测二氢杨梅素对4T1小鼠乳腺癌细胞的抗增殖作用；Transwell侵袭小室法检测二氢杨梅素对4T1细胞的抗转移作用。检测肿瘤的体积、远端肺转移灶数、荷瘤鼠血清及条件培养基中的基质金属蛋白酶MMP-9和MMP-2。结果二氢杨梅素处理4T1细胞48 h后，半数抑制浓度（IC50）为102.1μg/ml。二氢杨梅素能不依赖细胞毒作用减少细胞培养液中MMP-2和MMP-9水平，并且抑制4T1细胞的侵袭。与盐水对照组比较，100 mg·kg-1·d-1二氢杨梅素灌胃给药能显著减少瘤重、肺转移灶数以及荷瘤鼠血清 MMP-9和MMP-2水平；而50 mg·kg-1·d-1二氢杨梅素灌胃给药组除了血清MMP-2水平外，没有显著差异。结论二氢杨梅素给药能抑制4T1小鼠乳腺癌远端肺转移，其机制可能与二氢杨梅素下调荷瘤小鼠血清MMP-9和MMP-2水平相关。
目的：為瞭探討二氫楊梅素的抗轉移作用，在Balb/c雌性小鼠右後腿皮下建立瞭4T1乳腺癌移植模型，然後給予二氫楊梅素灌胃給藥。方法 MTT法檢測二氫楊梅素對4T1小鼠乳腺癌細胞的抗增殖作用；Transwell侵襲小室法檢測二氫楊梅素對4T1細胞的抗轉移作用。檢測腫瘤的體積、遠耑肺轉移竈數、荷瘤鼠血清及條件培養基中的基質金屬蛋白酶MMP-9和MMP-2。結果二氫楊梅素處理4T1細胞48 h後，半數抑製濃度（IC50）為102.1μg/ml。二氫楊梅素能不依賴細胞毒作用減少細胞培養液中MMP-2和MMP-9水平，併且抑製4T1細胞的侵襲。與鹽水對照組比較，100 mg·kg-1·d-1二氫楊梅素灌胃給藥能顯著減少瘤重、肺轉移竈數以及荷瘤鼠血清 MMP-9和MMP-2水平；而50 mg·kg-1·d-1二氫楊梅素灌胃給藥組除瞭血清MMP-2水平外，沒有顯著差異。結論二氫楊梅素給藥能抑製4T1小鼠乳腺癌遠耑肺轉移，其機製可能與二氫楊梅素下調荷瘤小鼠血清MMP-9和MMP-2水平相關。
목적：위료탐토이경양매소적항전이작용，재Balb/c자성소서우후퇴피하건립료4T1유선암이식모형，연후급여이경양매소관위급약。방법 MTT법검측이경양매소대4T1소서유선암세포적항증식작용；Transwell침습소실법검측이경양매소대4T1세포적항전이작용。검측종류적체적、원단폐전이조수、하류서혈청급조건배양기중적기질금속단백매MMP-9화MMP-2。결과이경양매소처리4T1세포48 h후，반수억제농도（IC50）위102.1μg/ml。이경양매소능불의뢰세포독작용감소세포배양액중MMP-2화MMP-9수평，병차억제4T1세포적침습。여염수대조조비교，100 mg·kg-1·d-1이경양매소관위급약능현저감소류중、폐전이조수이급하류서혈청 MMP-9화MMP-2수평；이50 mg·kg-1·d-1이경양매소관위급약조제료혈청MMP-2수평외，몰유현저차이。결론이경양매소급약능억제4T1소서유선암원단폐전이，기궤제가능여이경양매소하조하류소서혈청MMP-9화MMP-2수평상관。
Objective To investigates anti-metastasis effect of DMY, 4T1 mice breast carcinoma xenograft models were established in right hind leg subcutaneous of female Balb/c mice followed by DMY administration intragastrically. Methods Anti-proliferation and anti-metastasis effect of DMY were determined by the MTT assay and Transwell model, respectively. Tumor volume, distant pulmonary metastatic loci, and matrix metalloproteinases(MMP-9/MMP-2) levels in tumor-bearing mice serums and the conditioned media were analyzed. Results After treated by DMY for 48 h, IC50 value was about 102.1 μg/ml. DMY decreased MMP-9/MMP-2 levels in media and inhibited the invasion of 4T1 cells without cytotoxicity against the cancer cells. Compared with saline control group, 100 mg·kg-1·d-1 DMY decreased significantly tumor weight, lung metastatic loci and serum MMP-9/MMP-2 levels, while 50 mg·kg-1·d-1 DMY showed no significant difference except serum MMP-2 level. Conclusion DMY administration could suppress distant pulmonary metastasis of 4T1 mice breast carcinoma, and the mechanism was positively correlated with the down-regulation of tumor-bearing mice serum MMP-9/MMP-2 level by DMY.
