中华临床医师杂志(电子版)
中華臨床醫師雜誌(電子版)
중화림상의사잡지(전자판)
CHINESE JOURNAL OF CLINICIANS(ELECTRONIC VERSION)
2014年
10期
1894-1899
,共6页
李兰%王亚琦%王洪武%孙颖%黄加权%范翔雪%宁琴
李蘭%王亞琦%王洪武%孫穎%黃加權%範翔雪%寧琴
리란%왕아기%왕홍무%손영%황가권%범상설%저금
血吸虫病%肝硬化%细胞运动%肝星状细胞
血吸蟲病%肝硬化%細胞運動%肝星狀細胞
혈흡충병%간경화%세포운동%간성상세포
Schistosomiasis%Liver cirrhosis%Cell movement%Hepatic stellate cell
目的:探讨影响日本血吸虫病肝纤维化小鼠肝组织中肝星状细胞(HSCs)迁移运动功能变化的相关因素。方法 SPF级6~8周龄Balb/c小鼠16只,随机分为模型组(8只)和对照组(8只),以血吸虫尾蚴腹部贴附法建立感染模型,正常组予以生理盐水代替。于感染后8周末处理小鼠,取部分肝组织石蜡包埋,进行病理学评估,免疫荧光染色检测HSCs(α-SMA,红光)运动蛋白Fascin(绿光)的表达;另取部分肝组织,采用Real-time PCR方法检测迁移诱导因子转化生长因子β1(TGF-β1)、血小板源性生长因子(PDGF)以及单核细胞趋化因子1(MCP-1)的表达以及HSCs运动蛋白α-SMA、Fascin的表达。结果8周末时,模型组小鼠肝组织中已形成明显肝纤维化。模型组小鼠肝组织中TGF-β1、PDGF 以及MCP-1的基因表达水平分别是对照组的30倍、14倍及14倍,差异具有统计学意义(P=0.033、P=0.039以及P=0.037);同时,模型组中HSCs运动相关蛋白α-SMA和Fascin的基因表达水平分别是对照组的9倍和5倍,差异具有统计学意义(P=0.004、P=0.018);荧光共聚焦结果提示,模型组小鼠肝组织中α-SMA(红色)和Fascin(绿色)表达部位一致,集中在虫卵周围肝纤维化区域,较对照组二者表达明显增加,且红绿光分布多重叠。结论诱导HSCs运动迁移的因子表达增加和HSCs自身的运动相关蛋白表达增加均有利于HSCs运动迁移能力增强。
目的:探討影響日本血吸蟲病肝纖維化小鼠肝組織中肝星狀細胞(HSCs)遷移運動功能變化的相關因素。方法 SPF級6~8週齡Balb/c小鼠16隻,隨機分為模型組(8隻)和對照組(8隻),以血吸蟲尾蚴腹部貼附法建立感染模型,正常組予以生理鹽水代替。于感染後8週末處理小鼠,取部分肝組織石蠟包埋,進行病理學評估,免疫熒光染色檢測HSCs(α-SMA,紅光)運動蛋白Fascin(綠光)的錶達;另取部分肝組織,採用Real-time PCR方法檢測遷移誘導因子轉化生長因子β1(TGF-β1)、血小闆源性生長因子(PDGF)以及單覈細胞趨化因子1(MCP-1)的錶達以及HSCs運動蛋白α-SMA、Fascin的錶達。結果8週末時,模型組小鼠肝組織中已形成明顯肝纖維化。模型組小鼠肝組織中TGF-β1、PDGF 以及MCP-1的基因錶達水平分彆是對照組的30倍、14倍及14倍,差異具有統計學意義(P=0.033、P=0.039以及P=0.037);同時,模型組中HSCs運動相關蛋白α-SMA和Fascin的基因錶達水平分彆是對照組的9倍和5倍,差異具有統計學意義(P=0.004、P=0.018);熒光共聚焦結果提示,模型組小鼠肝組織中α-SMA(紅色)和Fascin(綠色)錶達部位一緻,集中在蟲卵週圍肝纖維化區域,較對照組二者錶達明顯增加,且紅綠光分佈多重疊。結論誘導HSCs運動遷移的因子錶達增加和HSCs自身的運動相關蛋白錶達增加均有利于HSCs運動遷移能力增彊。
목적:탐토영향일본혈흡충병간섬유화소서간조직중간성상세포(HSCs)천이운동공능변화적상관인소。방법 SPF급6~8주령Balb/c소서16지,수궤분위모형조(8지)화대조조(8지),이혈흡충미유복부첩부법건립감염모형,정상조여이생리염수대체。우감염후8주말처리소서,취부분간조직석사포매,진행병이학평고,면역형광염색검측HSCs(α-SMA,홍광)운동단백Fascin(록광)적표체;령취부분간조직,채용Real-time PCR방법검측천이유도인자전화생장인자β1(TGF-β1)、혈소판원성생장인자(PDGF)이급단핵세포추화인자1(MCP-1)적표체이급HSCs운동단백α-SMA、Fascin적표체。결과8주말시,모형조소서간조직중이형성명현간섬유화。모형조소서간조직중TGF-β1、PDGF 이급MCP-1적기인표체수평분별시대조조적30배、14배급14배,차이구유통계학의의(P=0.033、P=0.039이급P=0.037);동시,모형조중HSCs운동상관단백α-SMA화Fascin적기인표체수평분별시대조조적9배화5배,차이구유통계학의의(P=0.004、P=0.018);형광공취초결과제시,모형조소서간조직중α-SMA(홍색)화Fascin(록색)표체부위일치,집중재충란주위간섬유화구역,교대조조이자표체명현증가,차홍록광분포다중첩。결론유도HSCs운동천이적인자표체증가화HSCs자신적운동상관단백표체증가균유리우HSCs운동천이능력증강。
Objective To analyze the relevant changes of hepatic stellate cells (HSCs) migration in mice with Schistosoma japonicum infection. Methods A total of 16 SPF balb/c mice aged 6-8 weeks, were randomly divided into two groups, namely, control group (n=8) and infected group (n=8). The mice from the infected group were suffered from skin infection by schistosome cercariae, while the mice in control group were given an equal volume of saline instead. The mice were sacrificed at the end of the eighth week. Liver lesions were fixed immediately in 10% neutral-buffered formalin and processed into paraffin sections. Pathological changes and proliferation of hepatic collagen fibers in liver tissue were observed after HE staining and Masson staining. Changes in Fascin expression (green) of HSCs (red) were visualized by fluorescence staining and fluorescence images were recorded using confocal microscopy. By Real-time PCR, the expression of transforming growth factor-β1 (TGF-β1), platelet-derived growth factor (PDGF), monocyte chemotactic protein-1 (MCP-1), α-smooth muscle actin (α-SMA) and Fascin was analyzed. Results Visible liver fibrosis was observed in the infected group at the end of the eighth week. The mRNA levels of TGF-β1, PDGF, MCP-1,α-SMA and Fascin were more than 30-fold, 14-fold, 14-fold, 9-fold, and 5-fold higher in the infected group than in control group, respectively. The differences between two groups were statistically significant (P=0.033, P=0.039, P=0.037, P=0.004 and P=0.018, respectively). In addition, by confocal microscopy, Fascin immunoreactivity was almost undetectable in control group, but it was increased in the infected group along sinusoids and fibrosis area, overlapping with α-SMA, a maker of activated HSCs. Conclusion Enhancing the migration ability of activated HSCs which is a result of increasing expression of mobility-inducible factors and motility related proteins may play an important role in the progression of liver fibrosis infected by schistosoma japonicum.