CAJ | 학술논문

目的：探讨Th17细胞相关因子白细胞介素(IL)-17A、IL-17F、IL-21、IL-22与寻常性进行期银屑病发病的相关性。方法通过实时定量反转录聚合酶链式反应（RT-PCR）分别检测30例患者和20名正常人外周血单个核细胞（PBMC）、12例患者皮损、12名正常皮肤组织中上述4种细胞因子的mRNA表达水平。结果患者组PBMC中IL-17A、IL-17F、IL-21和IL-22的mRNA表达水平较正常组显著升高（P均＜0.05），患者组皮损中4种细胞因子的mRNA表达明显高于正常组（P均＜0.05）。结论 Th17细胞因子IL-17A、IL-17F、IL-21和IL-22的mRNA水平在患者组PBMC及皮肤组织中明显升高，提示Th17细胞因子可能与寻常性银屑病的发病有一定相关性。
목적：탐토Th17세포상관인자백세포개소(IL)-17A、IL-17F、IL-21、IL-22여심상성진행기은설병발병적상관성。방법통과실시정량반전록취합매련식반응（RT-PCR）분별검측30례환자화20명정상인외주혈단개핵세포（PBMC）、12례환자피손、12명정상피부조직중상술4충세포인자적mRNA표체수평。결과환자조PBMC중IL-17A、IL-17F、IL-21화IL-22적mRNA표체수평교정상조현저승고（P균＜0.05），환자조피손중4충세포인자적mRNA표체명현고우정상조（P균＜0.05）。결론 Th17세포인자IL-17A、IL-17F、IL-21화IL-22적mRNA수평재환자조PBMC급피부조직중명현승고，제시Th17세포인자가능여심상성은설병적발병유일정상관성。
ObjectiveTo investigate the relationship between Th17 cells related cytokines IL-17A, IL-17F, IL-21, IL-22 and psoriasis vulgaris.Methods Real-time RT-PCR was used to quantitatively detecte mRNA expressions of Th17-related cytokines IL-17A, IL-17F, IL-21 and IL-22 in PBMC of 30 patients and 20 normal humans, and in skin tissues of 12 cases of psoriasis vulgaris and 12 normal controls. Results The mRNA expressions of IL-17A, IL-17F, IL-21 and IL-22 were found signiifcantly higher in PBMC of patients compared with normal (P<0.05). Compared with normal controls, mRNA expressions of these four cytokines in psoriatic lesions increased (P<0.05). Conclusions The expression of Th17 related cytokines IL-17A, IL-17F, IL-21 and IL-22 mRNA increased signiifcantly in both PBMC and involved skin tissues of the psoriatic patients, which suggested that Th17 related cytokines might have some correlation with the pathogenesis of psoriasis vulgaris.