CAJ | 학술논문

目的：通过定量检测和比较 Attractin（Atrn） mRNA 和 Atrn蛋白在小鼠睾丸发育过程中的动态变化，为进一步研究Atrn在睾丸的生理作用提供实验依据。方法：用相对定量法检测 Atrn mRNA 在生后1、5、10、15、21、28、35、42、56和120天龄小鼠睾丸发育过程中的表达变化，并用Western blot 方法检测各天龄小鼠睾丸Atrn蛋白表达。结果：Atrn mRNA 和Atrn蛋白在小鼠睾丸发育过程中的表达变化趋势相似，在生后第1天两者均有表达，但是处于相对较低水平，此后是一个逐渐增高的过程，在28天出现较明显上调。在这之后，两者表达变化出现差异。 Atrn mRNA 在生后28～35天表达最丰富，随后稳定于这一水平，Atrn 蛋白表达则是继续增加直至成年。结论：Atrn参与精子发生的启动、精原干细胞的增殖分化、精母细胞的减数分裂和精子形成等过程，并有助于L ey dig 细胞进一步成熟。
목적：통과정량검측화비교 Attractin（Atrn） mRNA 화 Atrn단백재소서고환발육과정중적동태변화，위진일보연구Atrn재고환적생리작용제공실험의거。방법：용상대정량법검측 Atrn mRNA 재생후1、5、10、15、21、28、35、42、56화120천령소서고환발육과정중적표체변화，병용Western blot 방법검측각천령소서고환Atrn단백표체。결과：Atrn mRNA 화Atrn단백재소서고환발육과정중적표체변화추세상사，재생후제1천량자균유표체，단시처우상대교저수평，차후시일개축점증고적과정，재28천출현교명현상조。재저지후，량자표체변화출현차이。 Atrn mRNA 재생후28～35천표체최봉부，수후은정우저일수평，Atrn 단백표체칙시계속증가직지성년。결론：Atrn삼여정자발생적계동、정원간세포적증식분화、정모세포적감수분렬화정자형성등과정，병유조우L ey dig 세포진일보성숙。
Objectives :To study the role of Attractin (Atrn) in mouse testis ,mRNA and protein expres-sion of Atrn in mouse testis during post-natal development were investigated .Methods :The mRNA and pro-tein expression of Atrn in mouse testis during post-natal development (at day 1 ,5 ,10 ,15 ,21 ,28 ,35 ,42 ,56 and120 postpartum) were investigated by real time RT-PCR and Western blot separately .Results:The mR-NA and protein expression of Atrn had similar tendency in mouse testis during post-natal development .Rela-tive low level of Atrn mRNA and protein were detected in mouse testis on day 1 ,then increased obviously at day 28 .Expression of mRNA remained this level afterwards ,but protein expression increased gradually as the animal aged till maximal level in the adult testis .Conclusion:It was presumed that Atrn may be involved in spermatogenesis and Leydig cell development .