岭南现代临床外科
嶺南現代臨床外科
령남현대림상외과
LINGNAN MODERN CLINICS IN SURGERY
2014年
3期
247-251
,共5页
李颂%邓新军%李正明%李刚%阮黎%罗彬%黄海
李頌%鄧新軍%李正明%李剛%阮黎%囉彬%黃海
리송%산신군%리정명%리강%원려%라빈%황해
姜黄素%LY294002%前列腺癌%细胞凋亡
薑黃素%LY294002%前列腺癌%細胞凋亡
강황소%LY294002%전렬선암%세포조망
Curcumin%LY294002%Prostate cancer%Apoptosis
目的:探讨姜黄素与PI3K/Akt抑制剂LY294002联合应用对前列腺癌PC-3细胞体外生长的影响。方法根据给药不同将实验分为对照组、姜黄素组、LY294002组和姜黄素组+LY294002联合组,分别加入等量的培养基、25μmoL/L姜黄素、25μmoL/L的LY294002和两种混合液。采用MTS法检测各组细胞的增殖情况、流式细胞仪术检测各组细胞凋亡情况、q-PCR测定各组细胞中NF-κB、P53及caspase-9的表达情况。结果姜黄素组、LY组和联合组的细胞增值率均较对照组明显降低,且联合组明显低于两个单独用药组(P<0.05)。姜黄素与LY294002联合作用前列腺癌PC-3细胞后,细胞总凋亡率较姜黄素及LY294002单独使用后明显增加(P值均<0.05)。联合用药组的NF-κB表达量明显低于两单独用药组(P<0.05),而P53和caspase-9的表达量均明显高于两单独用药组(P<0.05)。结论姜黄素与LY294002联合使用较两种药物单独使用更能有效抑制前列腺癌PC-3细胞的体外生长,作用机制可能是通过抑制NF-κB的表达从而抑制细胞增殖,并增加P53和caspase-9的表达从而促进细胞凋亡来实现的。
目的:探討薑黃素與PI3K/Akt抑製劑LY294002聯閤應用對前列腺癌PC-3細胞體外生長的影響。方法根據給藥不同將實驗分為對照組、薑黃素組、LY294002組和薑黃素組+LY294002聯閤組,分彆加入等量的培養基、25μmoL/L薑黃素、25μmoL/L的LY294002和兩種混閤液。採用MTS法檢測各組細胞的增殖情況、流式細胞儀術檢測各組細胞凋亡情況、q-PCR測定各組細胞中NF-κB、P53及caspase-9的錶達情況。結果薑黃素組、LY組和聯閤組的細胞增值率均較對照組明顯降低,且聯閤組明顯低于兩箇單獨用藥組(P<0.05)。薑黃素與LY294002聯閤作用前列腺癌PC-3細胞後,細胞總凋亡率較薑黃素及LY294002單獨使用後明顯增加(P值均<0.05)。聯閤用藥組的NF-κB錶達量明顯低于兩單獨用藥組(P<0.05),而P53和caspase-9的錶達量均明顯高于兩單獨用藥組(P<0.05)。結論薑黃素與LY294002聯閤使用較兩種藥物單獨使用更能有效抑製前列腺癌PC-3細胞的體外生長,作用機製可能是通過抑製NF-κB的錶達從而抑製細胞增殖,併增加P53和caspase-9的錶達從而促進細胞凋亡來實現的。
목적:탐토강황소여PI3K/Akt억제제LY294002연합응용대전렬선암PC-3세포체외생장적영향。방법근거급약불동장실험분위대조조、강황소조、LY294002조화강황소조+LY294002연합조,분별가입등량적배양기、25μmoL/L강황소、25μmoL/L적LY294002화량충혼합액。채용MTS법검측각조세포적증식정황、류식세포의술검측각조세포조망정황、q-PCR측정각조세포중NF-κB、P53급caspase-9적표체정황。결과강황소조、LY조화연합조적세포증치솔균교대조조명현강저,차연합조명현저우량개단독용약조(P<0.05)。강황소여LY294002연합작용전렬선암PC-3세포후,세포총조망솔교강황소급LY294002단독사용후명현증가(P치균<0.05)。연합용약조적NF-κB표체량명현저우량단독용약조(P<0.05),이P53화caspase-9적표체량균명현고우량단독용약조(P<0.05)。결론강황소여LY294002연합사용교량충약물단독사용경능유효억제전렬선암PC-3세포적체외생장,작용궤제가능시통과억제NF-κB적표체종이억제세포증식,병증가P53화caspase-9적표체종이촉진세포조망래실현적。
Objective To explore the effect of curcumin in combined with LY294002 on prostate carcinoma PC-3 cells and the possible mechanism. Methods The experiments were divided into four groups according to different drug administration: control group (PPS), curcumin group (25 μmoL/L of curcumin), LY group (25 μmoL/L of LY294002) and combination group (mixture of curcumin and LY294002). Then proliferation viability of PC-3 cells was observed by MTS assay , apoptotic rate was detected by flow cytometry,.the mRNA expression of NF-κB、P53 and caspase-9 were examined by Q-PCR,respectively...Results....PC-3 cell proliferation was inhibited in three groups, curcumin group, LY294002 group and combination group. The proliferation rate in combination group was more decreased than that in other two groups..The apoptosis rate of PC-3 cells in combination group was significantly increased when compared with curcumin group or LY294002 group (P<0.05)..NF-κB expression in combination group was also significant lower than curcumin group or LY294002 group (P<0.05), while the expression of P53 and caspase-9 in combination group were obviously higher than these in groups of drug administrated alone (P<0.05). Conclusion The combined use of curcumin and LY294002 can effectively inhibit the growth of PC-3 cells in vitro , its mechanism might be inhibiting cell proliferation by decreasing the expression of NF-κB, and promoting cell apoptosis by increasing the expression of P53 and caspase-9.